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Comparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Medical Microbiology.
Malmö University Hospital.
Lund University.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Microbiology.
2011 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 84, no 2, 183-188 p.Article in journal (Refereed) Published
Abstract [en]

Molecular typing of Klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)5- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19 K. pneumoniae (some with an ESBL-phenotype), 35 K. oxytoca isolates, five K. pneumoniae, two K. oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)5 and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)5 and ITS-PCR analysis revealed that K. pneumoniae and K. oxytoca isolates, reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)5 and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates.

Place, publisher, year, edition, pages
Elsevier , 2011. Vol. 84, no 2, 183-188 p.
Keyword [en]
Klebsiella spp.; Molecular typing; (GTG)5-PCR; Ribosomal intergenic transcribed spacer (ITS)-PCR; Fingerprint analysis; Multiple displacement amplification
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-65401DOI: 10.1016/j.mimet.2010.11.019ISI: 000287951200005OAI: oai:DiVA.org:liu-65401DiVA: diva2:395449
Note
Original Publication: Anna Ryberg, Crister Olsson, Siv Ahrné and Hans-Jürg Monstein, Comparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates, 2011, Journal of Microbiological Methods, (84), 2, 183-188. http://dx.doi.org/10.1016/j.mimet.2010.11.019 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/Available from: 2011-02-07 Created: 2011-02-07 Last updated: 2017-12-11

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