Comparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates
2011 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 84, no 2, 183-188 p.Article in journal (Refereed) Published
Molecular typing of Klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)5- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19 K. pneumoniae (some with an ESBL-phenotype), 35 K. oxytoca isolates, five K. pneumoniae, two K. oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)5 and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)5 and ITS-PCR analysis revealed that K. pneumoniae and K. oxytoca isolates, reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)5 and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates.
Place, publisher, year, edition, pages
Elsevier , 2011. Vol. 84, no 2, 183-188 p.
Klebsiella spp.; Molecular typing; (GTG)5-PCR; Ribosomal intergenic transcribed spacer (ITS)-PCR; Fingerprint analysis; Multiple displacement amplification
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-65401DOI: 10.1016/j.mimet.2010.11.019ISI: 000287951200005OAI: oai:DiVA.org:liu-65401DiVA: diva2:395449
Original Publication: Anna Ryberg, Crister Olsson, Siv Ahrné and Hans-Jürg Monstein, Comparison of (GTG)5-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates, 2011, Journal of Microbiological Methods, (84), 2, 183-188. http://dx.doi.org/10.1016/j.mimet.2010.11.019 Copyright: Elsevier Science B.V., Amsterdam. http://www.elsevier.com/2011-02-072011-02-072012-03-23