liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A Collagen-Chitosan Hydrogel for Endothelial Differentiation and Angiogenesis
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Univ Ottawa, Inst Heart, Div Cardiac Surg, Ottawa, ON K1Y 4W7 Canada.
Show others and affiliations
2010 (English)In: TISSUE ENGINEERING PART A, ISSN 1937-3341, Vol. 16, no 10, 3099-3109 p.Article in journal (Refereed) Published
Abstract [en]

 Cell therapy for the treatment of cardiovascular disease has been hindered by low cell engraftment, poor survival, and inadequate phenotype and function. In this study, we added chitosan to a previously developed injectable collagen matrix, with the aim of improving its properties for cell therapy and neovascularization. Different ratios of collagen and chitosan were mixed and chemically crosslinked to produce hydrogels. Swell and degradation assays showed that chitosan improved the stability of the collagen hydrogel. In culture, endothelial cells formed significantly more vascular-like structures on collagen-chitosan than collagen-only matrix. While the differentiation of circulating progenitor cells to CD31(+) cells was equal on all matrices, vascular endothelial-cadherin expression was increased on the collagen-chitosan matrix, suggesting greater maturation of the endothelial cells. In addition, the collagen-chitosan matrix supported a significantly greater number of CD133(+) progenitor cells than the collagen-only matrix. In vivo, subcutaneously implanted collagen-chitosan matrices stimulated greater vascular growth and recruited more von Willebrand factor (vWF(+)) and CXCR4(+) endothelial/angiogenic cells than the collagen-only matrix. These results indicate that the addition of chitosan can improve the physical properties of collagen matrices, and enhance their ability to support endothelial cells and angiogenesis for use in cardiovascular tissue engineering applications.

Place, publisher, year, edition, pages
Mary Ann Liebert , 2010. Vol. 16, no 10, 3099-3109 p.
Keyword [en]
CIRCULATING PROGENITOR CELLS; EMBRYONIC STEM-CELLS; GROWTH-FACTOR; IN-VIVO; MYOCARDIAL-INFARCTION; CARDIAC REPAIR; RAT HEARTS; TRACKING; DELIVERY; BINDING
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-65511DOI: 10.1089/ten.tea.2009.0504OAI: oai:DiVA.org:liu-65511DiVA: diva2:396169
Available from: 2011-02-09 Created: 2011-02-09 Last updated: 2013-12-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Griffith, May

Search in DiVA

By author/editor
Griffith, May
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 92 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf