liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Optimal neural differentiation and extension of hybrid neuroblastoma cells (NDC) for nerve-target evaluations using a multifactorial approach
Ottawa Hosp, Res Inst, Ottawa, ON K1H 8L6 Canada.
Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON K1H 8M5 Canada .
Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON K1H 8M5 Canada .
Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON K1H 8M5 Canada .
Show others and affiliations
2010 (English)In: Toxicology in Vitro, ISSN 0887-2333, E-ISSN 1879-3177, Vol. 24, no 2, 567-577 p.Article in journal (Refereed) Published
Abstract [en]

In vitro models of tissues, such as the cornea, represent systems for modeling cell-to-cell interactions and tissue function. The objective of this study was to develop an optimized nerve differentiation medium to incorporate into a 3D in vitro model to study innervation and cell targeting. A hybrid neuroblastoma cell line (NDC) was examined for its ability to differentiate into neurons, produce neurites, and functionally contact target cells. Neuronal differentiation of NDCs was optimized through a combinatorial approach which involved culturing cells in the presence of various extracellular matrices and soluble factors. A serum-free medium containing nerve growth factor (NGF), dimethyl sulfoxide (DMSO), or dexamethasone resulted in the greatest proportion of NDCs demonstrating a neuronal morphology. Similarly, with supplementation of cyclic AMP (cAMP) or NGF, neurite extension was optimized. Combining these factors generated an optimized differentiation and extension medium, relative to the individual components alone. In co-culture with epithelial cells, NDC neurites generated in the optimized medium formed contacts with epithelial targets and produced substance P. Similarly, NDCs seeded into a collagen matrix produced neurites that projected through the matrix to target epithelial cells, promoted epithelial stratification, and increased the rate of epithelial wound healing. As well, differentiated NDCs could target and alter acetylcholine receptor clustering in mouse C2C12 myotubes, demonstrating synaptic plasticity. Our data supports the use of NDCs, in combination with optimized medium, for generating an innervated in vitro model. (C) 2009 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2010. Vol. 24, no 2, 567-577 p.
Keyword [en]
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-65515DOI: 10.1016/j.tiv.2009.10.016OAI: diva2:396184
Available from: 2011-02-09 Created: 2011-02-09 Last updated: 2013-12-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Griffith, May
In the same journal
Toxicology in Vitro
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 39 hits
ReferencesLink to record
Permanent link

Direct link