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A validated and rapid high-performance liquidchromatography method for the quantification ofconversion of radio-labelled sex steroids
Linköping University, Department of Clinical and Experimental Medicine, Oncology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medicine and Health Sciences, Clinical Pharmacology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medicine and Health Sciences, Clinical Pharmacology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Oncology. Linköping University, Faculty of Health Sciences.
2010 (English)In: Hormone Molecular Biology and Clinical Investigation, ISSN 1868-1891, Vol. 3, no 1, 375-381 p.Article in journal (Refereed) Published
Abstract [en]

The 17b -hydroxysteroid dehydrogenase enzymes modify the availability of potent sex steroids and have thus attracted interest in the study of several steroid-dependent pathologies including breast, endometrial and prostate cancers. An increased awareness of the importance of steroidogenic enzymes has brought forth a demand for efficient assays to study the effects of individual enzymes on steroid levels. Methods used for assessing steroid conversion are often laborious and frequently involve hazardous sample preparation steps. We developed and validated an optimised simple method for sample preparation of sex steroids using protein precipitation by the addition of zinc sulphate/sodium hydroxide. The interconversion of radio-labelled oestrogens and androgens was quantified using high-performance liquid chromatography separation of oestrone, oestradiol, androstenedione and testosterone followed by online radiometric flow scintillation analysis. The method, which can be applied for assessing, e.g., the efficacy of inhibitors of steroidogenic enzymes, was successfully used for evaluating oestrogenic interconversion in breast cancer cell lines MCF7 and T-47D.

Place, publisher, year, edition, pages
de Gruyter , 2010. Vol. 3, no 1, 375-381 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-65628DOI: 10.1515/HMBCI.2010.038OAI: oai:DiVA.org:liu-65628DiVA: diva2:397366
Available from: 2011-02-14 Created: 2011-02-14 Last updated: 2011-02-18

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Sivik, ToveVikingsson, SvanteGreén, HenrikJansson, Agneta

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