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Minimal residual disease assessment in childhood acute lymphoblastic leukaemia: a Swedish multi-centre study comparing real-time polymerase chain reaction and multicolour flow cytometry
Uppsala University.
Umea University.
Uppsala University.
Lund University.
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2011 (English)In: BRITISH JOURNAL OF HAEMATOLOGY, ISSN 0007-1048, Vol. 152, no 6, 743-753 p.Article in journal (Refereed) Published
Abstract [en]

Pandgt;Minimal residual disease (MRD) assessment is a powerful prognostic factor for determining the risk of relapse in childhood acute lymphoblastic leukaemia (ALL). In this Swedish multi-centre study of childhood ALL diagnosed between 2002 and 2006, the MRD levels were analysed in 726 follow-up samples in 228 children using real-time quantitative polymerase chain reaction (RQ-PCR) of rearranged immunoglobulin/T-cell receptor genes and multicolour flow cytometry (FCM). Using an MRD threshold of 0 center dot 1%, which was the sensitivity level reached in all analyses, the concordance between RQ-PCR and FCM MRD values at day 29 was 84%. In B-cell precursor ALL, an MRD level of andgt;= 0 center dot 1% at day 29 predicted a higher risk of bone marrow relapse (BMR) with both methods, although FCM was a better discriminator. However, considering the higher median MRD values achieved with RQ-PCR, a higher MRD cut-off (andgt;= 0 center dot 2%) improved the predictive capacity of RQ-PCR. In T-ALL, RQ-PCR was notably superior to FCM in predicting risk of BMR. That notwithstanding, MRD levels of andgt;= 0 center dot 1%, detected by either method at day 29, could not predict isolated extramedullary relapse. In conclusion, the concordance between RQ-PCR and FCM was high and hence both methods are valuable clinical tools for identifying childhood ALL cases with increased risk of BMR.

Place, publisher, year, edition, pages
Blackwell Publishing Ltd , 2011. Vol. 152, no 6, 743-753 p.
Keyword [en]
flow cytometry, RQ-PCR, rearranged IG, TCR genes, minimal residual disease, childhood acute lymphoblastic leukaemia
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-66314DOI: 10.1111/j.1365-2141.2010.08456.xISI: 000287739500008OAI: diva2:403223
Available from: 2011-03-11 Created: 2011-03-11 Last updated: 2012-01-17Bibliographically approved

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Department of Paediatrics in LinköpingPediatricsFaculty of Health Sciences
Engineering and Technology

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