A New Optical Method for Measuring Creatinine Concentration Removed During Dialysis
2010 (English)In: XII Mediterranean Conference on Medical and Biological Engineering and Computing 2010 IFMBE Proceedings, 2010, Springer , 2010, 379-382 p.Conference paper (Refereed)
The aim of this study was to compare creatinine (Cr) concentration measurements removed during dialysis by two optical algorithms based on single wavelength and multiwavelength UV-absorbance. Ten uremic patients, three females and seven males, mean age 62.6 ± 18.6 years, on chronic thrice-weekly hemodialysis were included in the study. Double-beam spectrophotometer (Shimatsu UV-2401 PC, Japan) was used for the determination of UV-absorbance in the collected spent dialysate samples. Two optical algorithms were developed to calculate Cr concentration removed during dialysis from measured UVabsorbance: (i) an algorithm utilizing only a single wavelength, revealing Cr concentration Cr_sw; (ii) an algorithm utilizing several wavelengths (multiwavelength algorithm), revealing Cr concentration Cr_mw. The mean value of Cr estimated at the laboratory was 107 ± 46,7 micromol/l, while UV-absorbance as Cr_sw (242 nm) was 107 ± 42.7 micromol/l, and 107 ± 44.7 micromol/l as Cr_mw. The mean concentrations were not significantly different (P = 0.99). The systematic errors, using Cr_lab as a reference, were -2.7% for Cr_sw and -1.7% for Cr_mw, and random errors were 17.3% and 13.6% for Cr_sw and Cr_mw, respectively. The systematic error was not significantly different for two optical algorithms (P = 0.25). The random error decreased significantly (P < 0.05) using Cr_mw algorithm compared to the Cr_sw model. In summary, the creatinine concentration removed during dialysis can be estimated with UV-absorbance technique.
Place, publisher, year, edition, pages
Springer , 2010. 379-382 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-66401DOI: 10.1007/978-3-642-13039-7_95ISBN: 978-3-642-13038-0 (print)ISBN: 978-3-642-13039-7 (online)OAI: oai:DiVA.org:liu-66401DiVA: diva2:403717
Biomedical Engineering (BME) Conference, 2010, 27-30 May in Porto Carras, Chalkidiki, Greece