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Are cutaneous microdialysis cytokine findings supported by end-point biopsy immunohistochemistry findings?
Linköping University, Department of Clinical and Experimental Medicine, Dermatology and Venerology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Dermatology and Venerology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Centre, Department of Dermatology and Venerology in Östergötland.
2010 (English)In: AAPS Journal, ISSN 1550-7416, E-ISSN 1550-7416, Vol. 12, no 4, 741-749 p.Article in journal (Refereed) Published
Abstract [en]

Insertion of a cutaneous microdialysis catheter into normal dermis has been shown to induce the production of IL1b, IL6 and IL8 in an innate response to minimal trauma. In the present study, skin biopsy for immunohistochemistry has been performed at the site of the microdialysis catheter to compare the findings with that of the microdialysis findings 24 h after insertion. Of the three named cytokines, concordance between the two investigated technologies was highest for IL8 (100%) followed by IL6 (70%) and IL1b (50%). For seven other pro-inflammatory and T cell-relevant cytokines studied, concordance ranged between 50% and 80%. The total number of positive (microdialysis or immunofluorescence) findings was similar between the two methodologies. Technical and biological phenomenon can explain the differences. We conclude that both methodologies illustrate important features of tissue biology and that a combination of the two methods in clinical research can provide the chronology of soluble mediator participation and the more classic, but also more invasive, biopsy-based methodology at a point which constitutes the end of the observation period. We conclude further that at the 24-h time period here studied, microdialysis catheters are still functional and thus capable of producing relevant data which can be corroborated and extended by the “end point biopsy”.

Place, publisher, year, edition, pages
2010. Vol. 12, no 4, 741-749 p.
Keyword [en]
cutaneous microdialysis, cytokines, human, immunostaining, innate
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-66928DOI: 10.1208/s12248-010-9235-8ISI: 000288426100028OAI: oai:DiVA.org:liu-66928DiVA: diva2:405379
Available from: 2011-03-22 Created: 2011-03-22 Last updated: 2017-12-11

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Sjögren, FlorenceAnderson, Chris D

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Dermatology and VenerologyFaculty of Health SciencesDepartment of Dermatology and Venerology in Östergötland
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