Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires
2011 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, Vol. 152, no 2, 241-247 p.Article in journal (Refereed) Published
In this study, a potentiometric uric acid biosensor was fabricated by immobilization of uricase onto zinc oxide (ZnO) nanowires. Zinc oxide nanowires with 80-150 nm in diameter and 900 nm to 1.5 mu m in lengths were grown on the surface of a gold coated flexible plastic substrate. Uricase was electrostatically immobilized on the surface of well aligned ZnO nanowires resulting in a sensitive, selective, stable and reproducible uric acid biosensor. The potentiometric response of the ZnO sensor vs Ag/AgCl reference electrode was found to be linear over a relatively wide logarithmic concentration range (1-650 mu M) suitable for human blood serum. By applying a Nafion (R) membrane on the sensor the linear range could be extended to 1-1000 mu M at the expense of an increased response time from 6.25 s to less than 9 s. On the other hand the membrane increased the sensor durability considerably. The sensor response was unaffected by normal concentrations of common interferents such as ascorbic acid, glucose, and urea.
Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2011. Vol. 152, no 2, 241-247 p.
ZnO nanowires, Potentiometric nanosensor, Uricase, Uric acid, Nafion (R), Membrane, Electrochemical nanodevices
National CategoryEngineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-67542DOI: 10.1016/j.snb.2010.12.015ISI: 000288877700015OAI: oai:DiVA.org:liu-67542DiVA: diva2:411267
Syed Usman Ali, Naveed Ul Hassan Alvi, Zafar Hussain Ibupoto, Omer Nur, Magnus Willander and Bengt Danielsson, Selective potentiometric determination of uric acid with uricase immobilized on ZnO nanowires, 2011, SENSORS AND ACTUATORS B-CHEMICAL, (152), 2, 241-247.
Copyright: Elsevier Science B.V., Amsterdam.