Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs
2011 (English)In: FORENSIC SCIENCE INTERNATIONAL-GENETICS, ISSN 1872-4973, Vol. 5, no 3, 194-198 p.Article in journal (Refereed) Published
Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas (R) Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5-32 mu L) with high or intermediate amylase activity (840 and 290 kU/L). Results were typically obtained within 5 min, and all samples that produced DNA profiles were positive. However, salivary amylase activities, as well as DNA concentrations, vary significantly between individuals. We show that there is no correlation between amylase activity and amount of DNA in fresh saliva. Even so, a positive amylase result indicates presence of saliva, and thereby presence of DNA. Amylase testing may be useful for screening in investigations where the number of DNA analyses is limited due to cost, e. g., in volume crime.
Place, publisher, year, edition, pages
ELSEVIER IRELAND LTD, ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND , 2011. Vol. 5, no 3, 194-198 p.
Amylase, Crime scene, Forensic DNA analysis, Presumptive test, Saliva, Sampling, Swab
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-67723DOI: 10.1016/j.fsigen.2010.03.003ISI: 000289193200019OAI: oai:DiVA.org:liu-67723DiVA: diva2:412647