Cationic polymer brush grafted-nanodiamond via atom transfer radical polymerization for enhanced gene delivery and bioimaging
2011 (English)In: JOURNAL OF MATERIALS CHEMISTRY, ISSN 0959-9428, Vol. 21, no 21, 7755-7764 p.Article in journal (Refereed) Published
A novel cationic nanodiamond-polymer brush was synthesized by 2-bromoisobutyrate-modified nanodiamond (ND) surface-initiated atomic transfer radical polymerization of 2-(dimethylamino)ethyl methacrylate (DMAEMA). Elemental analysis, FTIR, HRTEM, TGA and zeta potential analysis were used to confirm the successful synthesis of ND-polymer brushes. It was shown that the ND-brushes were capable of condensing plasmid DNA into stable nanoparticles, protecting DNA from enzyme degradation. Transfection studies demonstrated that the ND-brushes could not only efficiently deliver plasmids into COS-7 cells, but also mediate higher expression than PEI25k with lower cytotoxicity. The green fluorescence of ND-brushes could also be detected by laser scanning confocal microscopy, making the nanodiamond-polymer brushes an excellent multifunctional gene vector with not only high transfection efficiency but allowing for bioimaging.
Place, publisher, year, edition, pages
Royal Society of Chemistry , 2011. Vol. 21, no 21, 7755-7764 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-68787DOI: 10.1039/c1jm10813aISI: 000290670000033OAI: oai:DiVA.org:liu-68787DiVA: diva2:420868