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Detection of Bacterial Flora in Biological Secretions  Using Antibodies Developed In Vitro and Immobilized in a  Surface Plasmon Resonance System
Linköping University, Department of Physics, Chemistry and Biology, Molecular genetics .
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 60 credits / 90 HE creditsStudent thesis
Abstract [en]

Identification of pathogens living in biofilms of chronic infections has been difficult with PCR, serological, biochemical and culture techniques. The study aims at the detection of bacterial pathogens in biofilms of biological secretions using SPR analysis Biacore. The antibodies were developed by isolating mononuclear lymphocytes from the blood of the patients who sustained systemic infection. The isolated lymphocytes had antibody secreting B cells (plasma cells) which were identified using flow cytometry analysis. The antibodies produced (n=4) were used to immobilize CM5 chip of Biacore to detect the bacteria in ulcer secretions with wound secretions of healthy volunteers as controls. The results from Surface Plasmon Resonance (SPR) analysis and culture technique were compared and statistically there was no significant difference obtained. The results from present study suggest that SPR analysis could be used as an alternative system for detection of bacteria in poly-microbial samples and detect the organisms that might not be discovered by culture or PCR method.

Place, publisher, year, edition, pages
2011. , 14 p.
Keyword [en]
Biofilms, antibodies, lymphocytes, B cells, flow cytometry, immobilize, SPR analysis, Biacore, CM5 chip
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-69084ISRN: LiTH-IFM- Ex--2443--11OAI: diva2:423764
Subject / course
2011-05-27, 10:00
Available from: 2011-06-16 Created: 2011-06-16 Last updated: 2011-06-16Bibliographically approved

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