Molecular Fingerprint of Neuropeptide S-Producing Neurons in the Mouse Brain
2011 (English)In: JOURNAL OF COMPARATIVE NEUROLOGY, ISSN 0021-9967, Vol. 519, no 10, 1847-1866 p.Article in journal (Refereed) Published
Neuropeptide S (NPS) has been associated with a number of complex brain functions, including anxiety-like behaviors, arousal, sleep-wakefulness regulation, drug-seeking behaviors, and learning and memory. In order to better understand how NPS influences these functions in a neuronal network context, it is critical to identify transmitter systems that control NPS release and transmitters that are co-released with NPS. For this purpose, we generated several lines of transgenic mice that express enhanced green-fluorescent protein (EGFP) under control of the endogenous NPS precursor promoter. NPS/EGFP-transgenic mice show anatomically correct and overlapping expression of both NPS and EGFP. A total number of similar to 500 NPS/EGFP-positive neurons are present in the mouse brain, located in the pericoerulear region and the Kolliker-Fuse nucleus. NPS and transgene expression is first detectable around E14, indicating a potential role for NPS in brain development. EGFP-positive cells were harvested by laser-capture microdissection, and mRNA was extracted for expression profiling by using microarray analysis. NPS was found co-localized with galanin in the Kolliker-Fuse nucleus of the lateral parabrachial area. A dense network of orexin/hypocretin neuronal projections contacting pericoerulear NPS-producing neurons was observed by immunostaining. Expression of a distinct repertoire of metabotropic and ionotropic receptor genes was identified in both NPS neuronal clusters that will allow for detailed investigations of incoming neurotransmission, controlling neuronal activity of NPS-producing neurons. Stress-induced functional activation of NPS-producing neurons was detected by staining for the immediate-early gene c-fos, thus supporting earlier findings that NPS might be part of the brain stress response network.
Place, publisher, year, edition, pages
John Wiley and Sons, Ltd , 2011. Vol. 519, no 10, 1847-1866 p.
transgenic mice, green-fluorescent protein, microarray, gene expression, locus coeruleus, Kolliker-Fuse nucleus, galanin, development
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-69175DOI: 10.1002/cne.22603ISI: 000291111200002OAI: oai:DiVA.org:liu-69175DiVA: diva2:424311