liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Molecular Fingerprint of Neuropeptide S-Producing Neurons in the Mouse Brain
University of California Irvine.
University of California Irvine.
University of California Irvine.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Diagnostics, Department of Clinical Chemistry.
Show others and affiliations
2011 (English)In: JOURNAL OF COMPARATIVE NEUROLOGY, ISSN 0021-9967, Vol. 519, no 10, 1847-1866 p.Article in journal (Refereed) Published
Abstract [en]

Neuropeptide S (NPS) has been associated with a number of complex brain functions, including anxiety-like behaviors, arousal, sleep-wakefulness regulation, drug-seeking behaviors, and learning and memory. In order to better understand how NPS influences these functions in a neuronal network context, it is critical to identify transmitter systems that control NPS release and transmitters that are co-released with NPS. For this purpose, we generated several lines of transgenic mice that express enhanced green-fluorescent protein (EGFP) under control of the endogenous NPS precursor promoter. NPS/EGFP-transgenic mice show anatomically correct and overlapping expression of both NPS and EGFP. A total number of similar to 500 NPS/EGFP-positive neurons are present in the mouse brain, located in the pericoerulear region and the Kolliker-Fuse nucleus. NPS and transgene expression is first detectable around E14, indicating a potential role for NPS in brain development. EGFP-positive cells were harvested by laser-capture microdissection, and mRNA was extracted for expression profiling by using microarray analysis. NPS was found co-localized with galanin in the Kolliker-Fuse nucleus of the lateral parabrachial area. A dense network of orexin/hypocretin neuronal projections contacting pericoerulear NPS-producing neurons was observed by immunostaining. Expression of a distinct repertoire of metabotropic and ionotropic receptor genes was identified in both NPS neuronal clusters that will allow for detailed investigations of incoming neurotransmission, controlling neuronal activity of NPS-producing neurons. Stress-induced functional activation of NPS-producing neurons was detected by staining for the immediate-early gene c-fos, thus supporting earlier findings that NPS might be part of the brain stress response network.

Place, publisher, year, edition, pages
John Wiley and Sons, Ltd , 2011. Vol. 519, no 10, 1847-1866 p.
Keyword [en]
transgenic mice, green-fluorescent protein, microarray, gene expression, locus coeruleus, Kolliker-Fuse nucleus, galanin, development
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-69175DOI: 10.1002/cne.22603ISI: 000291111200002OAI: diva2:424311
Available from: 2011-06-17 Created: 2011-06-17 Last updated: 2011-06-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Theodorsson, Elvar
By organisation
Clinical ChemistryFaculty of Health SciencesDepartment of Clinical Chemistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 97 hits
ReferencesLink to record
Permanent link

Direct link