Mouse corneal lymphangiogenesis model.
2011 (English)In: Nature protocols, ISSN 1750-2799, Vol. 6, no 6, 817-26 p.Article in journal (Refereed) Published
This protocol describes a powerful in vivo method to quantitatively study the formation of new lymphatic vessels in the avascular cornea without interference of pre-existing lymphatics. Implantation of 100 ng of lymphangiogenic factors such as vascular endothelial growth factor (VEGF)-A, VEGF-C or fibroblast growth factor-2, together with slow-release polymers, into a surgically created micropocket in the mouse cornea elicits a robust lymphangiogenic response. Newly formed lymphatic vessels are detected by immunohistochemical staining of the flattened corneal tissue with lymphatic endothelial-specific markers such as lymphatic vessel endothelial hyaluronan receptor-1; less-specific markers such as vascular endothelial growth factor receptor 3 may also be used. Lymphatic vessel growth in relation to hemangiogenesis can be readily detected starting at day 5 or 6 after pellet implantation and persists for ∼14 d. This protocol offers a unique opportunity to study the mechanisms underlying lymphatic vessel formation, remodeling and function.
Place, publisher, year, edition, pages
2011. Vol. 6, no 6, 817-26 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-69194DOI: 10.1038/nprot.2011.359PubMedID: 21637201OAI: oai:DiVA.org:liu-69194DiVA: diva2:424448