liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Histonmodifieringar och alternativ splicing
Linköping University, Department of Physics, Chemistry and Biology.
2011 (Swedish)Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesisAlternative title
Histone modifications and alternative splicing (English)
Abstract [sv]

Alternativ splicing av pre-mRNA ger upphov till proteindiversitet. Histonmodifieringar kopplas till den alternativa splicingens reglering genom adaptorsystem som overfor den epigenetiska informationen direkt till splicingfaktorerna. De cis- agerande RNA- elementen pa exoner och introner med tillhorande trans- reglerande splicingfaktorer paverkas darfor direkt av specifika histonmodifieringar. En sammankopplande integrerad modell over en rad DNA- baserade processer foreslas. Denna komplexa modell ger en bild av interaktioner och paverkan mellan dessa delar. Kromatin remodellering kravs for bildandet av eukromatin. Nukleosomers placering vid exonrika regioner med specifika modifieringsmonster pekar ut exonerna samt mojliggor inbindning av RNA polymeras II som med sin CTD doman rekryterar bade splicing- och modifieringsfaktorer. Transkriptionshastigheten paverkas av nukleosomplaceringen vilket i sin tur paverkar rekrytering av spliceosomens komponenter, andra trans- agerande regulatorer och aven pre-mRNA sekvensens sekundarstruktur. Kromatin- adaptorkomplex laser av specifika histonmodifieringar och overfor informationen till splicingapparaten. Detta skapar mojlighet till den viktiga cell- och vavnadsspecifika alternativa splicingens reglering. I den integrerade modellen blir komplexiteten tydligare dar alla dessa processer interagerar med varandra och de cis- regulatoriska sekvenserna pa premRNA transkriptet.

Abstract [en]

Alternative splicing of pre-mRNA generates protein diversity. Histone modifications are connected to the regulation of alternative splicing through adaptor systems that transfers the epigenetic information directly to the splicing factors. The cis- acting RNA elements on the exons and introns together with the trans- regulating splicing factors are therefore directly affected of specific histone modifications. An integrated model over several DNA process mechanisms is suggested. This complex model explains the interactions of the different parts and how they affect each other. Chromatin remodelers are required to obtain euchromatin. Nucleosome positioning at exon rich regions with a specific modification pattern point out where the exons are, and this enable the RNA polymerase II to find and bind to the DNA. It’s CTD domain recruits both splicing- and modifications factors. The transcription rate is also affected of the nucleosome positioning and that in turn affects the recruitment of the components of the spliceosomen, other trans- acting regulators and even the formation of the secondary structure of the pre-mRNA transcript. Chromatin- adaptor complex reads specific histone modifications and transfers this information to the splicing apparatus. All this creates the possibility to regulate important cell- and tissue specific alternative splicing patterns. The integrated model makes the complex processes more clearer when all these integrates with each other and the cis- acting regulating elements on the pre-mRNA transcript.

Place, publisher, year, edition, pages
2011. , 22 p.
Keyword [en]
Alternative splicing, Histone modifications, exon, intron, pre-mRNA splicing, splicing regulation, chromatin- adaptor system, integrated model
Keyword [sv]
Alternativ splicing, Histonmodifieringar, exon, intron, pre-mRNA splicing, splicing regulering, kromatin- adaptor system, integrerad modell
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:liu:diva-69679ISRN: LiTH-IFM- EX--11/2498--SEOAI: oai:DiVA.org:liu-69679DiVA: diva2:431033
Subject / course
Biochemistry
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2011-08-08 Created: 2011-07-14 Last updated: 2011-08-08Bibliographically approved

Open Access in DiVA

fulltext(747 kB)1020 downloads
File information
File name FULLTEXT01.pdfFile size 747 kBChecksum SHA-512
6b5a2d705fd95264bce8f6e8b7487c01e9abeb94fe7d65dd4878f613cb89a1fa69ea67bd60030730db04ccb370da0b69ca8a7dd35b671919037a3e4eb22ba2ae
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Berggren, Jenny
By organisation
Department of Physics, Chemistry and Biology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 1020 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 207 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf