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Characterization of new medium-chain alcohol dehydrogenases adds resolution to duplications of the class I/III and the sub-class I genes
Karolinska Institute.
Linköping University, Department of Physics, Chemistry and Biology, Bioinformatics . Linköping University, The Institute of Technology.
Karolinska Institute.
Karolinska Institute.
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2011 (English)In: Chemico-Biological Interactions, ISSN 0009-2797, E-ISSN 1872-7786, Vol. 191, no 03-janArticle in journal (Refereed) Published
Abstract [en]

Four additional variants of alcohol and aldehyde dehydrogenases have been purified and functionally characterized, and their primary structures have been determined. The results allow conclusions about the structural and evolutionary relationships within the large family of MDR alcohol dehydrogenases from characterizations of the pigeon (Columba livia) and dogfish (Scyliorhinus canicula) major liver alcohol dehydrogenases. The pigeon enzyme turns out to be of class I type and the dogfish enzyme of class III type. This result gives a third type of evidence, based on purifications and enzyme characterization in lower vertebrates, that the classical liver alcohol dehydrogenase originated by a gene duplication early in the evolution of vertebrates. It is discernable as the major liver form at about the level in-between cartilaginous and osseous fish. The results also show early divergence within the avian orders. Structures were determined by Edman degradations, making it appropriate to acknowledge the methodological contributions of Pehr Edman during the 65 years since his thesis at Karolinska Institutet, where also the present analyses were performed.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2011. Vol. 191, no 03-jan
Keyword [en]
Gene duplications; Vertebrate enzyme evolution; Alcohol dehydrogenase; Liver enzyme origin; Avian order taxonomy
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-69905DOI: 10.1016/j.cbi.2011.02.006ISI: 000291767200004OAI: diva2:433284
Available from: 2011-08-09 Created: 2011-08-08 Last updated: 2011-08-09

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Hedlund, JoelPersson, Bengt
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