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Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease
Lund University.
Lund University.
University and Reg Labs Reg Shane.
Linköping University, Department of Medical and Health Sciences, Pharmacology. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Centre, Department of Nephrology UHL.
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2011 (English)In: Journal of Cystic Fibrosis, ISSN 1569-1993, E-ISSN 1873-5010, Vol. 10, no 4, 265-271 p.Article in journal (Refereed) Published
Abstract [en]

The clinical consequence of chronic Pseudomonas aeruginosa colonization in cystic fibrosis (CF) varies between individuals for unknown reasons. Auto-antibodies against bactericidal/permeability increasing protein (BPI-ANCA) are associated with poor prognosis in CF. We hypothesize that there is a correlation between the presence of BPI-ANCA, the properties of the colonizing bacteria and the clinical conditions of the host. We compared isolates of P. aeruginosa from BPI-ANCA positive CF patients who have deteriorating lung disease with BPI-ANCA negative CF patients who are in stable clinical conditions. Epithelial cells (A549) and isolated polymorphonuclear granulocytes (PMNs) were stimulated with the isolates and cell death was analyzed with flow cytometry. We found that the ANCA associated strains in most cases showed pyocyanin negative phenotypes. These strains also induced less inflammatory response than the non-ANCA associated strains as shown by apoptosis and necrosis of epithelial cells and neutrophils. Our results suggest that colonization with strains of P. aeruginosa that induce a weak inflammatory response is associated with unfavorable outcome in CF. We speculate that inadequate control of pathogen proliferation through an insufficient inflammatory response results in a slowly increasing number of bacteria and accumulation of dying PMNs in the airways, contributing to progression in CF lung disease.

Place, publisher, year, edition, pages
Elsevier Science B.V., Amsterdam. , 2011. Vol. 10, no 4, 265-271 p.
Keyword [en]
BPI-ANCA; Pseudomonas aeruginosa; Chronic colonization; Neutrophils; Pyocyanin; Interleukin 8; Anti-neutrophil cytoplasmic antibodies; Apoptosis
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-69833DOI: 10.1016/j.jcf.2011.03.004ISI: 000292065900007OAI: diva2:433468
Available from: 2011-08-10 Created: 2011-08-08 Last updated: 2011-12-08

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Segelmark, Mårten
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PharmacologyFaculty of Health SciencesDepartment of Nephrology UHL
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