Effect of Different Extenders and Seminal Plasma on the Susceptibility of Equine Spermatozoa to Lipid Peroxidation After Single-Layer Centrifugation, Through Androcoll-E
2011 (English)In: Journal of Equine Veterinary Science, ISSN 0737-0806, E-ISSN 1542-7412, Vol. 31, no 7, 411-416 p.Article in journal (Refereed) Published
his study was conducted in an attempt to see whether single-layer centrifugation (SLC) increases the susceptibility of stallion spermatozoa to lipid peroxidation (LPO), in different extenders after removing all seminal plasma (SP). The susceptibility of stallion spermatozoa to LPO was studied before and after SLC. Each ejaculate was split, and aliquots extended with one of the three different extenders: INRA 96, Kenneys, or Equipro, and stored for 24 hours at 5 degrees C (i). From the extended samples, an aliquot was kept as a control and the other was subjected to SLC through Androcoll-E. The selected spermatozoa were re-suspended in the appropriate extenders, without (ii) or with (iii) addition of 50% (v/v) pooled homologous SP for 24 hours at 5 degrees C. Using ferrous sulfate as pro-oxidant, the susceptibility for LPO was flow-cytometrically assessed using the probe Bodipy(581/591)-C(11). Sperm motility, monitored with a Qualisperm motility analyzer, increased after SLC treatment (P andlt; .001). No significant correlations were found between motility and induced LPO with ferrous sulfate. The SP and extenders, per se, did not have a significant protective effect against LPO, but the interaction between SP and Kenney increased the susceptibility to LPO. However, the selected spermatozoa through Androcoll-E and the subsequent dilution in INRA had a significant protective effect against LPO (P andlt; .05), especially when the oxidative insults were higher (80 mu M).
Place, publisher, year, edition, pages
Elsevier Science B. V., Amsterdam , 2011. Vol. 31, no 7, 411-416 p.
Lipid peroxidation, Equine, Extenders, Seminal plasma, Single-layer centrifugation, Motility
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-69990DOI: 10.1016/j.jevs.2011.01.010ISI: 000293311400009OAI: oai:DiVA.org:liu-69990DiVA: diva2:433995
Original Publication: Cristina Ortega-Ferrusola, Anders Johannisson, Fernando J Pena Vega, Jose A Tapia, Heriberto Rodriguez-Martinez, Ann M Dalin and Jane M Morrell, Effect of Different Extenders and Seminal Plasma on the Susceptibility of Equine Spermatozoa to Lipid Peroxidation After Single-Layer Centrifugation, Through Androcoll-E, 2011, Journal of Equine Veterinary Science, (31), 7, 411-416. http://dx.doi.org/10.1016/j.jevs.2011.01.010 Copyright: WB Saunders http://www.elsevier.com/2011-08-122011-08-122015-03-12