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Phadebas® Press test and the presence of amylases in different body fluids deposited on textile
Linköping University, Department of Physics, Chemistry and Biology, Molecular genetics . Linköping University, The Institute of Technology.
Swedish National Laboratory of Forensic Science (SKL), Linköping, Sweden.
Swedish National Laboratory of Forensic Science (SKL), Linköping, Sweden.
Swedish National Laboratory of Forensic Science (SKL), Linköping, Sweden.
2011 (English)In: Book of Abstracts, 2011, 136- p.Conference paper (Refereed)
Abstract [en]

In forensic DNA casework saliva stains with epithelial cells can be very useful even presenting the key evidence.Tests for amylase activity, like Phadebas® Press test, help locate stains and indicate presence of saliva. Sensitivityis high, with positive amylase tests obtained prior to detectable levels of DNA and saliva diluted to 1:100readily generate a positive reaction with Phadebas® Press test for presence of amylase. The salivary amylaseactivity varies on individual basis over time as well as it does between individuals. In addition some individualssecrete high levels of amylases [1,2]. However, amylases are present in other body fluids as well, generally toomuch lower levels than saliva. Due to sensitivity of amylase tests there is a potential interference by otherfluids when using them to verify the presence of saliva. Other studies also demonstrate that e.g. faeces can givepositive reactions.For underwear the presence of several different body fluids might have natural causes, including vaginal secretions,(menstrual) blood, urine, faeces, as well as semen and saliva. Here we present the use of Phadebas® Presstest on underwear with naturally deposited body fluids and single source body fluid mock samples including oneindividual with higher levels of amylase activity. Our results and implications are discussed.

[1] J. Hedman, E. Dalin, B. Rasmusson, R. Ansell (2011). Forensic Science International; Genetics, 5, 194–198.

[2] J. Hedman, K. Gustavsson, R. Ansell (2008). Forensic Science International; Genetics Supplement Series, 1(1), 430–432.

Place, publisher, year, edition, pages
2011. 136- p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-70276OAI: diva2:437718
24th World Congress of the International Society for Forensic Genetics 2011
Available from: 2011-08-30 Created: 2011-08-30 Last updated: 2011-09-05

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Molecular genetics The Institute of Technology
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