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Mechanical stimulation of epithelial cells using polypyrrole microactuators.
Karolinska Institutet, Swedish Medical Nanoscience Center, Department of Neuroscience, Stockholm, Sweden.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.ORCID iD: 0000-0001-5154-0291
Karolinska Institutet, Swedish Medical Nanoscience Center, Department of Neuroscience, Stockholm, Sweden.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-2071-7768
2011 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 11, no 19, 3287-3293 p.Article in journal (Refereed) Published
Abstract [en]

The importance of mechanotransduction for physiological systems is becoming increasingly recognized. The effect of mechanical stimulation is well studied in organs and tissues, for instance by using flexible tissue culture substrates that can be stretched by external means. However, on the cellular and subcellular level, dedicated technology to apply appropriate mechanical stimuli is limited. Here we report an organic electronic microactuator chip for mechanical stimulation of single cells. These chips are manufactured on silicon wafers using traditional microfabrication and photolithography techniques. The active unit of the chip consists of the electroactive polymer polypyrrole that expands upon the application of a low potential. The fact that polypyrrole can be activated in physiological electrolytes makes it well suited as the active material in a microactuator chip for biomedical applications. Renal epithelial cells, which are responsive to mechanical stimuli and relevant from a physiological perspective, are cultured on top of the microactuator chip. The cells exhibit good adhesion and spread along the surface of the chip. After culturing, individual cells are mechanically stimulated by electrical addressing of the microactuator chip and the response to this stimulation is monitored as an increase in intracellular Ca(2+). This Ca(2+) response is caused by an autocrine ATP signalling pathway associated with mechanical stimulation of the cells. In conclusion, the present work demonstrates a microactuator chip based on an organic conjugated polymer, for mechanical stimulation of biological systems at the cellular and sub-cellular level.

Place, publisher, year, edition, pages
2011. Vol. 11, no 19, 3287-3293 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-70442DOI: 10.1039/c1lc20436jISI: 000294503200011PubMedID: 21842071OAI: oai:DiVA.org:liu-70442DiVA: diva2:439479
Note

Funding Agencies|Swedish Foundation for Strategic Research (OBOE Strategic Research Center for Organic Bioelectronics)||VINNOVA||Knut and Alice Wallenberg Foundation||Royal Swedish Academy of Science||Onnesjo Foundation||

Available from: 2011-09-07 Created: 2011-09-07 Last updated: 2017-12-08Bibliographically approved

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Berggren, MagnusJager, Edwin W H

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