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Properties of poly-aminophenylboronate coatings in capillary electrophoresis for the selective separation of diastereoisomers and glycoproteins
Department of Science and Technology, University of Verona, Verona, Italy.
Department of Science and Technology, University of Verona, Verona, Italy.
Institute of BioScience and Technology, Cranfield University at Silsoe, Silsoe, Bedfordshire, UK.
Cranfield University, UK.ORCID iD: 0000-0002-1815-9699
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2004 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1023, no 2, 297-303 p.Article in journal (Refereed) Published
Abstract [en]

The polymerisation of 3-aminophenylboronic acid (APBA) in aqueous environment has been used for the open tubular modification of capillary electrophoresis (CE) capillaries. Being poly-APBA endowed with boronic acid, aromatic rings and secondary amines groups, it posses a variety of functional groups affecting selectivity. Diastereoisomers (e.g. ascorbic and isoascorbic acid) and proteins (e.g. haemoglobins) were successfully separated onto poly-APBA column, by means of a combination of electrophoresis and open tubular electrochromatography. The mechanism of selection was investigated: results indicate an interplay between enhancing or silencing the contribution of the protonable functionahties (amino groups, boronic acid). The properties of APBA polymer coating make it attractive for CE separation and for further application in affinity separations and chip technologies.

Place, publisher, year, edition, pages
Elsevier , 2004. Vol. 1023, no 2, 297-303 p.
Keyword [en]
coated columns; diastereomer separation; aminophenylboronic acid; glycoproteins; ascorbic acid; hemoglobins
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-70604DOI: 10.1016/j.chroma.2003.10.023ISI: 000187720100015OAI: diva2:440897
Available from: 2011-09-14 Created: 2011-09-14 Last updated: 2013-10-04

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Turner, Anthony P. F.
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