liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Biotin-specific synthetic receptors prepared using molecular imprinting
Institute of BioScience and Technology, Cranfield Centre for Analytical Science, Cranfield University, Cranfield, Bedfordshire, UK.
Institute of BioScience and Technology, Cranfield Centre for Analytical Science, Cranfield University, Cranfield, Bedfordshire, UK.
Institute of BioScience and Technology, Cranfield Centre for Analytical Science, Cranfield University, Cranfield, Bedfordshire, UK.
Institute of BioScience and Technology, Cranfield Centre for Analytical Science, Cranfield University, Cranfield, Bedfordshire, UK.
Show others and affiliations
2004 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 504, no 1, 179-183 p.Article in journal (Refereed) Published
Abstract [en]

The composition of new molecularly imprinted polymers (MIPs) specific for biotin was optimised using molecular modelling software. Three functional monomers: methacrylic acid (MAA), 2-(trifluoromethyl)acrylic acid (TFAA) and 2-acrylamido-2-methylpropanesulfonic acid (AMPSA), which demonstrated the highest binding scores with biotin, were tested on their ability to generate specific binding sites. The imprinted polymers were photografted to the surface of polystyrene microspheres in water. The affinity of the synthetic "receptor" sites was evaluated in binding experiments using horseradish peroxidase-labelled biotin. Good correlation was found between the modelling results and the performance of the materials in the template re-binding study. The dissociation constants for all MIPs were 1.4-16.8 nM, which is sufficient for most analytical applications where biotin is used as a label.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2004. Vol. 504, no 1, 179-183 p.
Keyword [en]
biotin; computational design; molecularly imprinted polymer; photografting polymerisation
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-70603DOI: 10.1016/S0003-2670(03)00813-4ISI: 000188705000022OAI: oai:DiVA.org:liu-70603DiVA: diva2:440899
Available from: 2011-09-14 Created: 2011-09-14 Last updated: 2017-12-08

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Turner, Anthony

Search in DiVA

By author/editor
Turner, Anthony
In the same journal
Analytica Chimica Acta
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 142 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf