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Localization of candidate stem and progenitor cell markers within the human cornea, limbus, and bulbar conjunctiva in vivo and in cell culture
University of Ottawa Eye Institute, Ottawa, Ontario, Canada.
University of Ottawa Eye Institute, Ottawa, Ontario, Canada.ORCID iD: 0000-0003-1222-6720
2006 (English)In: Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology, ISSN 1552-4884, E-ISSN 1932-8494, Vol. 288A, no 8, 921-931 p.Article in journal (Refereed) Published
Abstract [en]

Corneal diseases are some of the most prevalent causes of blindness worldwide. While the most common treatment for corneal blindness is the transplantation of cadaver corneas, expanded limbal stem cells are finding recent application. Unknown, however, is the identity of the actual repopulating stem cell fraction utilized in both treatments and the critical factors governing successful engraftment and repopulation. In order to localize potential stem cell populations in vivo, we have immunohistochemically mapped a battery of candidate stem and progenitor cell markers including c-Kit and other growth factor receptors, nuclear markers including Delta Np63, as well as adhesion factors across the cornea and distal sclera. Cell populations that differentially and specifically stained for some of these markers include the basal and superficial limbal/conjunctival epithelium and scattered cells within the substantia propria of the bulbar conjunctiva. We have also determined that the culture of differentiated cornea epithelial cells as dissociated and explant cultures induces the expression of several markers previously characterized as candidate limbal stem cell markers. This study provides a foundation to explore candidate corneal stem cell populations. As well, we show that expression of traditional stem cell markers may not be reliable indicator of stem cell content during limbal stem cell expansion in vitro and could contribute to the variable success rates of corneal stem cell transplantation.

Place, publisher, year, edition, pages
John Wiley and Sons, Ltd , 2006. Vol. 288A, no 8, 921-931 p.
Keyword [en]
stem cell; progenitor cell; limbus; bulbar conjunctiva; immunohistochemistry; cell culture
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-70681DOI: 10.1002/ar.a.20346ISI: 000239473500010OAI: oai:DiVA.org:liu-70681DiVA: diva2:441023
Available from: 2011-09-14 Created: 2011-09-14 Last updated: 2017-12-08

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Griffith, May

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