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Immunological responses in mice to full-thickness corneal grafts engineered from porcine collagen
Department of Ophthalmology, Institute of Medical Science, Foresterhill, University of Aberdeen, Aberdeen, Scotland, UK.
Department of Ophthalmology, Institute of Medical Science, Foresterhill, University of Aberdeen, Aberdeen, Scotland, UK.
University of Ottawa Eye Institute, Ottawa Health Research Institute-Vision Centre, Ottawa, ON, Canada.ORCID iD: 0000-0003-1222-6720
Department of Ophthalmology, Institute of Medical Science, Foresterhill, University of Aberdeen, Aberdeen, Scotland, UK.
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2007 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 28, no 26, 3807-3814 p.Article in journal (Refereed) Published
Abstract [en]

Tissue-engineered (TE) corneas were fabricated from porcine collagen cross-linked with 1-ethyl-3-(3-dimethyl aminoproplyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS), and were transplanted into BALB/c mice orthotopically using a full-thickness penetrating keratoplasty (PKP) procedure. The biocompatibility was evaluated by assessing both local and systemic immune responses. Myeloid cells including granulocytes and macrophages were the main infiltrating cells in recipient cornea and in retro-TE corneal membrane which developed 7-10 days post surgery. Sodium citrate was found to be effective in reducing fibrin accumulation in anterior chamber post grafting at early time points, but it did not prevent formation of the retro-TE corneal membrane. No significant T cell activation was observed in the submandibular draining lymph nodes (SMDLN) by flow cytometry. Anti-porcine type I collagen IgG antibodies were detected in the serum of grafted mice from 2 weeks post grafting and the concentration of antibodies increased with time. Overall, porcine collagen-EDC/NHS TE corneas were tolerated well in murine recipients, causing mainly a self-limiting local innate immune response and a low-grade humoral response with little evidence of sustained T cell activation. Retro-TE corneal membrane formation was the main complication and barrier to clarity.

Place, publisher, year, edition, pages
Elsevier , 2007. Vol. 28, no 26, 3807-3814 p.
Keyword [en]
porcine collagen; tissue-engineered cornea; keratoplasty; immunology
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-70676DOI: 10.1016/j.biomaterials.2007.04.025ISI: 000248875300005OAI: oai:DiVA.org:liu-70676DiVA: diva2:441031
Available from: 2011-09-14 Created: 2011-09-14 Last updated: 2017-12-08

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