Enterovirus 70 binds to different glycoconjugates containing alpha 2,3-linked sialic acid on different cell lines
2005 (English)In: Journal of Virology, ISSN 0022-538X, E-ISSN 1098-5514, Vol. 79, no 11, 7087-7094 p.Article in journal (Refereed) Published
Enterovirus 70 (EV70), the causative agent of acute hemorrhagic conjunctivitis, exhibits a restricted tropism for conjunctival and corneal cells in vivo but infects a wide spectrum of mammalian cells in culture. Previously, we demonstrated that human CD55 is a receptor for EV70 on HeLa cells but that EV70 also binds to sialic acid-containing receptors on a variety of other human cell lines. Virus recognition of sialic acid attached to underlying glycans by a particular glycosidic linkage may contribute to host range, tissue tropism, and pathogenesis. Therefore, we tested the possibility that EV70 binds to andalpha; 2,3-linked sialic acid, like other viruses associated with ocular infections. Through the use of linkage-specific sialidases, sialyltransferases, and lectins, we show that EV70 recognizes andalpha; 2,3-linked sialic acid on human corneal epithelial cells and on U-937 cells. Virus attachment to both cell lines is CD55 independent and sensitive to benzyl N-acetyl-andalpha;-D-galactosaminide, an inhibitor of O-linked glycosylation. Virus binding to corneal cells, but not U-937 cells, is inhibited by proteinase K, but not by phosphatidylinositol-specific phospholipase C treatment. These results are consistent with the idea that a major EV70 receptor on corneal epithelial cells is an O-glycosylated, non-glycosyl phosphatidylinositol-anchored membrane glycoprotein containing andalpha; 2,3-linked sialic acid, while sialylated receptors on U-937 cells are not proteinaceous.
Place, publisher, year, edition, pages
American Society for Microbiology , 2005. Vol. 79, no 11, 7087-7094 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-70672DOI: 10.1128/JVI.79.11.7087-7094.2005ISI: 000229085400053OAI: oai:DiVA.org:liu-70672DiVA: diva2:441035