liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Collagen-poly(N-isopropylacrylamide)-based membranes for corneal stroma scaffolds
Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital Cornea Center, Ichikawa, Chiba, Japan.
Laval University Medical Center (CHUL), Laval University, Quebec, Canada.
University of Ottawa Eye Institute, University of Ottawa, Ottawa, Ontario, Canada.ORCID iD: 0000-0003-1222-6720
Santen Pharmaceutical Co. Ltd., Nara R&D Center, Ikoma-shi, Nara, Japan.
Show others and affiliations
2003 (English)In: Cornea, ISSN 0277-3740, E-ISSN 1536-4798, Vol. 22, no 7, S81-S88 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: To investigate the feasibility of using the biocompatibility of collagen-based blended biomaterials as cell-delivery systems in ocular surface reconstruction in vivo. Methods: Collagen-based composites that were blended with synthetic acrylamide-based polymers [poly(N-isopropylacrylamide), pNIPAAm] were transplanted into corneal pockets of white rabbits, with a 3-mm epithelial window. Epithelial cells were allowed to migrate onto the polymer. Transplanted eyes were examined daily for up to 30 days, after which animals; were killed for histologic examination. lm- munohistochemistry was performed for vimentin, a-smooth muscle actin (alpha-SMA), CD4, and CD8. Gold-chloride staining was performed to observe neuronal regrowth. Human amniotic membranes (AMs) and sham-operated corneas served as controls. All animals received topical antibiotics (levofloxacin) without the use of steroids or other immunosuppressive agents. Results: The pNIPAAm polymer allowed smooth epitheliatization of the cornea, which was similar to the epithelialization observed in sham controls and AM-transplanted eyes. Histology revealed that epithelium overlying the polymer was bundled into several layers, without the orientation observed with AM and sham controls. The polymer gradually thinned and was gradually replaced by host tissue. Vimentin- and alpha-SMA-positive cells were found in stromal pockets up to 1 month following polymer transplantation. These cells were responsible for slight subepithelial haze near the wound edge. CD4- and CD8-positive lymphocytes were also observed in the vicinity of the polymer. Gold-chloride staining showed nerve regrowth in the wound edge after 1 month and subepithelial branches after 3 months. Conclusion: Collagen-pNIPAAm blended polymers may he effective as biomaterials to be used in the early stages of lamellar stromal replacement.

Place, publisher, year, edition, pages
Lippincott, Williams andamp; Wilkins , 2003. Vol. 22, no 7, S81-S88 p.
Keyword [en]
collagen; cornea; isopropylacrylamide; keratoplasty; polymer
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-70668DOI: 10.1097/00003226-200310001-00012ISI: 000185654300012OAI: diva2:441044
Available from: 2011-09-14 Created: 2011-09-14 Last updated: 2013-12-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Griffith, May
In the same journal
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 40 hits
ReferencesLink to record
Permanent link

Direct link