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A collagen-based scaffold for a tissue engineered human cornea: Physical and physiological properties
CHUL Research Center, Laval University, Quebec City, Quebec, Canada.
Department of Physiology, University of Tennessee, Health Science Center, Memphis, TN, USA.
University of Ottawa Eye Institute, University of Ottawa, Ottawa, Canada.
Department of Physiology, University of Tennessee, Health Science Center, Memphis, TN, USA.
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2003 (English)In: International Journal of Artificial Organs, ISSN 0391-3988, E-ISSN 1724-6040, Vol. 26, no 8, 764-773 p.Article in journal (Refereed) Published
Abstract [en]

Stabilized collagen-glycosaminoglycan scaffolds for tissue engineered human corneas were characterized. Hydrated matrices were constructed by blending type I collagen with chondroitin sulphates (CS), with glutaraldehyde crosslinking. A corneal keratocyte cell line was added to the scaffolds with or without corneal epithelial and endothelial cells. Constructs were grown with or without ascorbic acid. Wound-healing was evaluated in chemical-treated constructs. Native, noncrosslinked gels were soft with limited longevity. Crosslinking strengthened the matrix yet permitted cell growth. CS addition increased transparency. Keratocytes grown within the matrix had higher frequencies of K+ channel expression than keratocytes grown on plastic. Ascorbic acid increased uncrosslinked matrix degradation in the presence of keratocytes, while it enhanced keratocyte growth and endogenous collagen synthesis in crosslinked matrices. Wounded constructs showed recovery from exposure to chemical irritants. In conclusion, this study demonstrates that our engineered, stabilized matrix is well-suited to function as an in vitro corneal stroma.

Place, publisher, year, edition, pages
Wichtig Editore , 2003. Vol. 26, no 8, 764-773 p.
Keyword [en]
artificial cornea; extracellular matrix; keratocyte; wound healing; tissue engineering; ascorbic acid
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-70659ISI: 000185616900010OAI: oai:DiVA.org:liu-70659DiVA: diva2:441079
Available from: 2011-09-14 Created: 2011-09-14 Last updated: 2017-12-08

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Griffith, M

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