Tissue-engineered injectable collagen-based matrices for improved cell delivery and vascularization of ischemic tissue using CD133+progenitors expanded from the peripheral blood
2006 (English)In: Circulation, ISSN 0009-7322, E-ISSN 1524-4539, Vol. 114, I138-I144 p.Article in journal (Refereed) Published
Background-The use of stem and/or progenitor cells to achieve potent vasculogenesis in humans has been hindered by low cell numbers, implant capacity, and survival. This study investigated the expansion of CD133(+) cells and the use of an injectable collagen-based tissue engineered matrix to support cell delivery and implantation within target ischemic tissue. Methods and Results-Adult human CD133(+) progenitor cells from the peripheral blood were generated and expanded by successive removal and culture of CD133(-) cell fractions, and delivered within an injectable collagen-based matrix into the ischemic hindlimb of athymic rats. Controls received injections of phosphate-buffered saline, matrix, or CD133(+) cells alone. Immunohistochemistry of hindlimb muscle 2 weeks after treatment revealed that the number of CD133(+) cells retained within the target site was greater than 2-fold greater when delivered by matrix than when delivered alone (P less than 0.01). The transplanted CD133(+) cells incorporated into vascular structures, and the matrix itself also was vascularized. Rats that received matrix and CD133(+) cells demonstrated greater intramuscular arteriole and capillary density than other treatment groups (P less than 0.05 and P less than 0.01, respectively). Conclusions-Compared with other experimental approaches, treatment of ischemic muscle tissue with generated CD133(+) progenitor cells delivered in an injectable collagen-based matrix significantly improved the restoration of a vascular network. This work demonstrates a novel approach for the expansion and delivery of blood CD133(+) cells with resultant improvement of their implantation and vasculogenic capacity.
Place, publisher, year, edition, pages
LIPPINCOTT WILLIAMS and WILKINS , 2006. Vol. 114, I138-I144 p.
angiogenesis; cells; endothelial progenitor cells; ischemia; revascularization; tissue engineering
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-70695DOI: 10.1161/CIRCULATIONAHA.105.001081ISI: 000238688200024OAI: oai:DiVA.org:liu-70695DiVA: diva2:441085