Stress-induced alternative splicing of Serine/Arginine-rich proteins in the moss Physcomitrella patens
Independent thesis Advanced level (degree of Master (Two Years)), 40 credits / 60 HE creditsStudent thesis
Plants are sessile organisms and thus more exposed to stressful environments. By changing the expression of stress related genes, plants are able to cope with stress. Alternative splicing (AS) of pre-mRNA is a major contributor to proteome diversity in eukaryotes. It has been shown that different abiotic stresses affect AS patterns, suggesting a functional role of AS in stress tolerance. The Serine/Arginine-rich proteins (SR proteins) are a conserved family of splicing regulators in eukaryotes. SR proteins are essential for AS and studies have shown that they are themselves subjects to AS after stress exposure which means that they can control their own splicing. In this study, the aim was to characterize the different SR-proteins in the SR subfamily in P. patens, analyze their phylogeny and measure the change in expression of the genes after exposure to five types of stress; osmotic, salinity, dehydration, cold and hormonal. The result showed both individual and overlapping changes in their expression profiles of the three genes. Furthermore, there was an alteration in the alternative splicing pattern for two genes during three of the stresses which resulted in intron retention and possibly a premature termination codon and subseqent non-sense mediated decay.
Place, publisher, year, edition, pages
2011. , 23 p.
Alternative splicing, Physcomitrella patens, Serine/Arginine-rich proteins, Stress
Genetics Bioinformatics and Systems Biology
IdentifiersURN: urn:nbn:se:liu:diva-71258ISRN: LITH-IFM-A-EX--11/2444--SEOAI: oai:DiVA.org:liu-71258DiVA: diva2:446489
Subject / course
Programme for Teacher Education, Linköping
UppsokLife Earth Science