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On-line control of glucose feeding in an Escherichia coli fed-batch cultivation expressing a recombinant protein.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology .
2011 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Soft sensors have been suggested as potent tools for on-line estimations of critical bioprocess variables to be able to control the biological process in an as high extent as possible. The formation of inhibitory by-products in the form of organic acids, caused by an overflow of glucose, is a problem in most bioprocesses expressing recombinant proteins. 

 

In this project a new method of controlling the glucose feeding in an Escherichia coli fed-batch cultivation expressing the green fluorescent protein (GFP) was investigated. The new controller system implemented in the software controlled the feed rate based on on-line HPLC measurements of the concentration of organic acids.   

 

The results showed that the controller managed to down-regulate the inhibitory organic acids to a low level as it tried to keep the glucose uptake rate at an optimum for maximum cell growth. The results suggested that the controller could be a powerful tool to create a more secure reproducibility and to generate high product yields in recombinant protein productions.   

Place, publisher, year, edition, pages
2011. , 43 p.
Keyword [en]
Soft sensors, Escherichia coli, Green fluorescent protein, Overflow metabolism and mixed acid fermentation, Glucose regulation
National Category
Bioprocess Technology
Identifiers
URN: urn:nbn:se:liu:diva-71305ISRN: LITH-IFM-A-EX-11/2561-SEOAI: oai:DiVA.org:liu-71305DiVA: diva2:447209
Subject / course
Biotechnology
Uppsok
Technology
Supervisors
Examiners
Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2011-10-11Bibliographically approved

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On-line control of glucose feeding in an Escherichia coli fed-batch cultivation expressing a recombinant protein.(2185 kB)1105 downloads
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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf