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Investigation of the phototoxic effect of ZnO nanorods on fibroblasts and melanoma human cells
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
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2014 (English)In: Laser Physics Letters, ISSN 1612-2011, E-ISSN 1612-202X, Vol. 11, no 11, 115606Article in journal (Refereed) Published
Abstract [en]

Photo-cytotoxicity of zinc oxide (ZnO) nanowires (NWs) either bare or conjugated with photosensitizers was studied in dark and after ultraviolet light exposure, in human melanoma and foreskin fibroblast cells. ZnO NWs were grown on the capillary tip and then coated with photosensitizer. This coated tip was used as pointer for intracellular insertion of ZnO NWs and photosensitizer. ZnO NWs pointer was inserted into a specific cell and then irradiated with ultraviolet (UVA), which led to loss of mitochondrial membrane potential, as estimated by loss of the Mitotracker Red staining. Dissolved ZnO NWs showed cytotoxicity as detected by MTT viability assay and morphological evaluation. UVA-irradiation enhanced the toxicity and caused the production of reactive oxygen species (ROS) resulting in cell necrosis. ZnO NWs were photo-toxic for both normal and cancer cells, questioning their bio-safety.

Place, publisher, year, edition, pages
Institute of Physics (IOP), 2014. Vol. 11, no 11, 115606
Keyword [en]
ZnO nanowires, photodynamic therapy, reactive oxygen species, δ- Aminolevulinic acid, protoporphyrin 1X
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-71316DOI: 10.1088/1612-2011/11/11/115606OAI: oai:DiVA.org:liu-71316DiVA: diva2:447260
Note

The previous status of this article was Manuscript and the working title was Photo toxicity of Zinc Oxide Nanowires in Human Melanoma and Fibroblast Cells.

Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2017-08-30Bibliographically approved
In thesis
1. Device Fabrication and Photosensitizing Role of ZnO Nanostructures in Photodynamic Therapy of Cancer
Open this publication in new window or tab >>Device Fabrication and Photosensitizing Role of ZnO Nanostructures in Photodynamic Therapy of Cancer
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In nanoscience and nanotechnology, zinc oxide (ZnO) is gaining much research attention due to direct wide band gap (3.3 eV), large exciton binding energy (60 meV), and deep level defects emissions that cover the whole visible range. ZnO nanorods (NRs) in comparison to normal bio molecules and large surface area to volume ratio, allow them to interact within the cell thus are used as convincing intracellular carriers of photosensitizers. Vertical NRs are wave guiding cavities enhancing the light extraction efficiency from devices and are stable photosensitizing agents with their biophotonic, and biodegradation properties, therefore are appealing candidates for the photodynamic therapy of cancer.

The heterojunction LEDs of ZnO NRs/p-GaN are best choice to take the advantage of GaN ideal blue-light emission and fabricated LEDs explore the potential of white LEDs with superior performance. The main objective of this thesis is not only to fabricate ZnO NRs/p-GaN, or ZnO nanotubes (ZNTs)/p-GaN heterostructures, but also to investigate their optical properties for photodynamic therapy. These LEDs have showed enhanced EL intensity covering the visible band (425–750 nm).

ZnO nanorods are grown on the borosilicate glass capillaries (0.7 μm diameter) and then conjugated with photosensitizer. Such glass capillaries having ZnO nanorods complex with photosensitizer on them are used as pointer for intracellular mediated photochemistry in cells to achieve their necrosis. Mitochondrial staining of melanoma and foreskin fibroblast cells was done by Mitotracker Red with the aim of targeting the specific organelle with the prepared ZnO nanowires (NWs) Femtotip to see ROS production. Cytotoxic effects of nanometallic oxides e.g. ZnO-NRs, MnO2 NRs, and Fe2O3 NPs individually and their ligands with photosensitizers in osteosarcoma (U2OS) cells are also explored. Thus bare and ligands of nanometallic oxides, with particular focus of ZnO nanowires are having significant and convincing cytotoxic effects via the liberation of reactive oxygen species as well as Zn+2 ions in labeled cells, thus can be assigned as anticancer agents for breast cancer, melanoma cancer and osteosarcoma cells.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. 56 p.
Series
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1392
Keyword
Zinc oxide nanostructures, light emitting diodes, reactive oxygen species, photosensitizer, cancer cell, photodynamic therapy
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-71319 (URN)978-91-7393-083-3 (ISBN)
Public defence
2011-10-21, K2, Kåkenhus, Campus Norrköping, Linköpings universitet, Linköping, 10:15 (English)
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Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2014-01-15Bibliographically approved

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Kishwar, SultanaIsrar, Muhammad QadirNour, OmerWillander, MagnusÖllinger, Karin

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