Biotoxicity of nanometallic oxides and their ligands with photosensitizers in osteosarcom a cells
(English)Manuscript (preprint) (Other academic)
The cytotoxic effects in osteosarcoma (U2OS) cells to different nanosized metallic oxides e.g. zinc oxide nanowires (ZnO-NWs), manganese di-oxide nanowires (MnO2 NWs), ferric oxide nanoparticles (Fe2O3 NPs) individually and their complex forms with photosensitizers photofrin®, 5-Aminolevulinic acid (5-ALA), and protoporphyrin IX (Pp IX) were studied. The cellular effects were assayed by analyzing the cellular morphology. The reactive oxygen species (ROS) were detected using 2', 7'-Dichlorofluorescein diacetate, and cell viability were assessed using MTT assay under ultraviolet (UV), visible light and laser exposed conditions. Prominent cell death with above cited nanomaterials in their complex forms with photosensitizer was observed in labeled U2OS cells. This cell death might be due to their synergetic effect via the release of singlet oxygen species in osteosarcoma cells showing their anticancer-cell effects.
Osteosarcoma cell, reactive oxygen species (ROS), MTT assay, photodynamic therapy (PDT)
IdentifiersURN: urn:nbn:se:liu:diva-71317OAI: oai:DiVA.org:liu-71317DiVA: diva2:447261