Structural characterization and biocompatible applications of graphene nanosheets for miniaturization of potentiometric cholesterol biosensor
2011 (English)In: Journal of Biosensors & Bioelectronics, ISSN 2155-6210, Vol. 2, no 3Article in journal (Refereed) Published
The potentiometric cholesterol biosensor based on graphene nanosheets has been successfully miniaturized. Cholesterol oxidase (ChOx) has been immobilized onto graphene nanosheets exfoliated on copper wire through the process of physical adsorption,. The presented potentiometric biosensor renders effective selectivity and sensitivity (~82 mV/decade) for the detection of cholesterol biomolecules in 1 × 10−6 M to 1 × 10−3 M logarithmic range and quick output response within ~ 4 sec. The stability and reusability of the biosensor has also been investigated for the above mentioned range of cholesterol concentrations. The enzyme activity measurements on graphene nanosheets are studied using UV-Visible and FTIR spectrophotometers. Additionally, the functioning of the presented biosensor is studied for a range of temperatures (15-70 °C) and pH values (4-9).
Place, publisher, year, edition, pages
2011. Vol. 2, no 3
Graphene nanosheets; Cholesterol; Potentiometric; Biosensor
IdentifiersURN: urn:nbn:se:liu:diva-71322DOI: 10.4172/2155-6210.1000109OAI: oai:DiVA.org:liu-71322DiVA: diva2:447371
On the day of the defence day the status of this arcile was "Manuscript".2011-10-112011-10-112014-11-24Bibliographically approved