Electroactive Control of Platelet Adhesion to Conducting Polymer Micropatterns
(English)Manuscript (preprint) (Other academic)
In this work, we report a method to fabricate addressable micropatterns of electroactive surfaces based on the conducting polymer poly-(3, 4-ethylenedioxytiophene) (PEDOT:Tos) to gain dynamic control over the spatial distribution of platelets, in vitro. Utilizing thin film processing and microfabrication techniques desired patterns down to the scale of individual cells, were achieved to enable active regulation of cell populations and their extracellular environment at high spatial resolution.Upon electronic addressing, both reduced and oxidized surfaces were created within the same device. The changes of the electrochemical state of PEDOT results in a reversible modification of the surface properties of the material. This surface modulation dictates the conformation and/or orientation, rather than the concentration, of surface proteins, thus indirectly regulating cell adhesion. The chemistry, texture, charge, and softness of fiacrtiial cell-hosting surfaces are parameters known to affect the binding characteristics and orientation of the extracellular proteins, thus dictating adhesion, spreading, migration, and proliferation of cells.
IdentifiersURN: urn:nbn:se:liu:diva-71360OAI: oai:DiVA.org:liu-71360DiVA: diva2:447697