liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
New Methods for Selecting Stallion Spermatozoa for Assisted Reproduction
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Medical Genetics.ORCID iD: 0000-0002-5194-2124
Show others and affiliations
2011 (English)In: Journal of Equine Veterinary Science, ISSN 0737-0806, E-ISSN 1542-7412, Vol. 31, no 9, 536-541 p.Article in journal (Refereed) Published
Abstract [en]

Improved sperm selection techniques are needed to increase the efficiency of equine-assisted reproduction. Single layer centrifugation (SLC) of spermatozoa has been shown to improve the quality of stallion sperm samples. In this study, the functionality of selected stallion spermatozoa was tested by intracytoplasmic sperm injection of equine oocytes after selection by SLC through Androcoll-E or by discontinuous density gradient centrifugation (DGC) through Redigrad and Tyrodes medium with added albumin, lactate, and pyruvate. The mean cleavage rates of the injected oocytes from SLC- and DGC-selected spermatozoa were 67% and 66%, respectively, whereas the proportion of blastocysts developing from cleaved oocytes was 28% and 22%, respectively (P andgt; .05, not significant). An incidental finding was that there was a tendency for SLC-selected spermatozoa to have a higher percentage of spermatozoa with normal morphology than DGC (70% +/- 22% vs. 58% +/- 38%) and for more blastocysts to be obtained from subfertile ejaculates (21 [19.6%] vs. 15 [14.4%], respectively). In further experiments, stallion spermatozoa bound to hyaluronan, although binding may depend on the semen extender and sperm treatment as well as incubation time. In conclusion, SLC-selected stallion spermatozoa function normally when injected into oocytes. SLC may potentially be better than DGC at selecting spermatozoa from subfertile ejaculates, but this effect needs rigorous investigation with a much larger sample size. Use of the hyaluronan-binding assay for assessing the potential fertility of stallion spermatozoa may be useful but requires further evaluation.

Place, publisher, year, edition, pages
WB Saunders , 2011. Vol. 31, no 9, 536-541 p.
Keyword [en]
ICSI, Stallion spermatozoa, Hyaluronan binding, Androcoll-E, Single layer centrifugation
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-71386DOI: 10.1016/j.jevs.2011.03.009ISI: 000295117300008OAI: diva2:448047
|Available from: 2011-10-14 Created: 2011-10-14 Last updated: 2015-03-12

Open Access in DiVA

fulltext(316 kB)866 downloads
File information
File name FULLTEXT01.pdfFile size 316 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
By organisation
Faculty of Health SciencesMedical Genetics
In the same journal
Journal of Equine Veterinary Science
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 866 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 164 hits
ReferencesLink to record
Permanent link

Direct link