Orientation and capturing of antibody affinity ligands: Applications to surface plasmon resonance biochips
2011 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, Vol. 158, no 1, 265-270 p.Article in journal (Refereed) Published
A surface plasmon resonance (SPR) sensor chip with immobilized protein G was used for simultaneously capturing, purifying and orienting antibody ligands. The ligands were further stabilized by chemical cross-linking. This procedure of designing the sensor chip improved efficient use of the ligands and could prolong the analytical use. less thanbrgreater than less thanbrgreater thanThe procedure was evaluated on standard dextran-coated sensor chips onto which commercial semi-purified antibodies towards human serum albumin and human troponin where captured and used for analysing their antigens. less thanbrgreater than less thanbrgreater thanThe procedure demonstrates a general design approach for presenting the biorecognition element on a biosensor surface which enhances sensitivity, stability and selectivity at the same time as an impure ligand is purified.
Place, publisher, year, edition, pages
Elsevier , 2011. Vol. 158, no 1, 265-270 p.
Biosensor, Affinity interaction, SPR, Biacore, Protein G, Sensor chip
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-71770DOI: 10.1016/j.snb.2011.06.017ISI: 000295500200037OAI: oai:DiVA.org:liu-71770DiVA: diva2:453964
Funding Agencies|European Commission|LSHB-CT-2007-037636|2011-11-042011-11-042015-05-28