liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Combined type 1 diabetes and celiac disease in children give raise to a pronounced Treg population
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics. Linköping University, Faculty of Health Sciences.
Clinic of Paediatrics, Ryhov County Hospital, Jönköping, Sweden 4.
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping.
Linköping University, Department of Clinical and Experimental Medicine, Pediatrics. Linköping University, Faculty of Health Sciences.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Regulatory T-cells (Treg) suppress the immune response in order to avoid harmful effects. A wide range of highly expressed markers are associated to Treg, among them are CD4, CD25, CD39 and forkhead box p3 (FOXP3). Others are expressed in a low number on Tregs, for example CD45RA and CD127. Type 1 diabetes (T1D) and celiac disease are both autoimmune diseases, caused by an unwanted immune response, and Treg cells have been associated to both diseases. As T1D and celiac disease share same risk genes, patients have the risk of developing the other disease subsequently. Treg cells have been implicated to be associated with development of T1D combined with celiac disease.

This pilot study aimed to investigate the expression of Treg associated markers in both CD4+CD25+ and CD4+CD25high cells by flow cytometry. In order to evaluate the involvement of Treg cells, CD39, CD45RA, CD127 and FOXP3 were studied in children with combination of T1D and celiac disease, in comparison to children with either T1D or celiac disease, and healthy children.

Our data point out that children with combination of T1D and celiac disease have higher expression of FOXP3 as well as CD39, together with a decreased expression of both CD127 and CD45RA, in comparison to children with exclusively T1D or celiac disease. Even though none of the groups differed from the reference group, our data indicates that children with combination of T1D and celiac disease have a more pronounced Treg population, both in frequency and MFI, compared to children with either T1D or celiac disease.

National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-71912OAI: oai:DiVA.org:liu-71912DiVA: diva2:455271
Available from: 2011-11-09 Created: 2011-11-09 Last updated: 2011-11-09Bibliographically approved
In thesis
1. Combinations of type 1 diabetes, celiac disease and allergy: An immunological challenge
Open this publication in new window or tab >>Combinations of type 1 diabetes, celiac disease and allergy: An immunological challenge
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The immune system is composed of a complex network of different cell types protecting the body against various possible threats. Among these cells are T-helper (Th) cells type 1 (Th1) and type 2 (Th2), as well as T regulatory (Treg) cells. Th1 and Th2 are supposed to be in balance with each other, while Tregs regulate the immune response, by halting it when the desired effect, i.e. destroying the threat, is acquired. However, sometimes this intricate interplay in the immune system is disturbed, leading to diseases as type 1 diabetes (T1D), celiac disease or allergic disease. According to the paradigm claiming that Th1- and Th2-cells inhibit each other a coexistence of a Th1-deviated disease and a Th2-deviated disease seems unlikely.

This thesis aimed to examine the immune response with focus on subsets of T-cells in children with T1D, celiac disease, allergy, or a combination of two of these diseases, in comparison to reference children (healthy).

In line with previous findings we observed that children with celiac disease showed a decreased spontaneous Th2-associated secretion, whereas children with allergic disease showed increased birch- and cat-induced Th2-associated response.

The most remarkable results in this thesis are those observed in children with combinations of diseases. The combination of T1D and celiac disease decreased the Th1-associated response against several antigens, but instead displayed a more pronounced Treg-associated response. Further, in children with combined T1D and allergy an increased Th1- and Th2-associated response was seen to a general stimulus, and an increased birch-induced Th1-, Th2-, Treg- and pro-inflammatory response. In contrast, the combination of allergy and celiac disease showed a decreased spontaneous Th1-, Th2-, Treg- and pro-inflammatory response.

In conclusion, we observed that two Th1-deviated diseases in combination suppress the immune response and increase the regulatory activity. Further it seems that allergy has the ability to shift the immune response in diverging directions depending on which disease it is combined with. The observed suppressive effect might be due to exhaustion of the immune system from the massive pressure of two immunological diseases in combination, while the pronounced Treg response might be caused by an attempt to compensate for the dysfunction. These results shed some light on the intriguing and challenging network that constitutes the immune system, and hopefully give clues regarding disease prevention and treatment.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2011. 109 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1277
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-71915 (URN)978-91-7393-021-5 (ISBN)
Public defence
2011-12-02, Berzeliussalen, Hälsouniversitetet, Campus US, Linköpings universitet, Linköping, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2011-11-09 Created: 2011-11-09 Last updated: 2011-11-17Bibliographically approved

Open Access in DiVA

No full text

Authority records BETA

Kivling, AnnaNilsson, LennartFaresjö, Maria

Search in DiVA

By author/editor
Kivling, AnnaNilsson, LennartFaresjö, Maria
By organisation
PediatricsFaculty of Health SciencesDepartment of Paediatrics in Linköping
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 68 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf