Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability
2011 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 127, no 1-2, 78-83 p.Article in journal (Refereed) Published
Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p andlt; 0.05) and velocity (p andlt; 0.05), membrane integrity (increases ranging from 11 to 14%, p andlt; 0.05) and mitochondrial membrane potential (p andlt; 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual.
Place, publisher, year, edition, pages
Elsevier Masson , 2011. Vol. 127, no 1-2, 78-83 p.
Stallion, Frozen sperm, Flow cytometry, CASA
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-72147DOI: 10.1016/j.anireprosci.2011.07.009ISI: 000295765400012OAI: oai:DiVA.org:liu-72147DiVA: diva2:457496
Funding Agencies|Ministerio de Ciencia e Innovacion, Madrid, Spain|INIA R22008-00018-00-00AGL 2010-20758 GAN|Junta de Extremadura FEDER|GR 10010|2011-11-182011-11-182015-03-12