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Study of protein adsorption on structured surfaces using ellipsometry
Linköping University, Department of Physics, Chemistry and Biology.
2011 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

In order to measure the thickness of a protein layer on a structured surface of silicon rubber, we have used ellipsometry and Fourier transform infrared (FTIR)-spectroscopy. The aim was to determine whether this type of measurement method can be used on protein layers or not. By hot-embossing a specific pattern of micrometre-sized pillars was created on the surface of the silicon rubber, which then was exposed to a phosphate buffer solution (PBS) containing human serum albumin (HSA) protein. FTIR measurements confirmed that proteins had attached to the surface. Ellipsometric studies were made and even though the protein layer was too thin to be measured, a simulation was made that revealed that a protein layer needs to be at least 1,5 nm to be measured properly with this method. We can also see that the protein molecules can get out of the solution, to find their way into the small pits of the samples.

Place, publisher, year, edition, pages
2011. , 37 p.
Keyword [en]
Ellipsometry, FTIR-spectroscopy, protein adsorption, hydrophobicity
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-72165ISRN: LITH-IFM-G-EX--11/2526--SEOAI: oai:DiVA.org:liu-72165DiVA: diva2:457757
Subject / course
Physics
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2011-11-21 Created: 2011-11-19 Last updated: 2012-01-05Bibliographically approved

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf