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NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase (TDH1)
Chalmers University of Technology.
Göteborg University.
National Laboratory of Forensic Science,.
Chalmers University of Technology.
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2004 (English)In: Current Genetics, ISSN 0172-8083, E-ISSN 1432-0983, Vol. 45, no 2, 90-95 p.Article in journal (Refereed) Published
Abstract [en]

A strain of Saccharomyces cerevisiae lacking the GPD2 gene, encoding one of the glycerol-3-phosphate dehydrogenases, grows slowly under anaerobic conditions, due to reductive stress caused by the accumulation of cytoplasmic NADH. We used 2D-PAGE to study the effect on global protein expression of reductive stress in the anaerobically grown gpd2 strain. The most striking response was a strongly elevated expression of Tdh1p, the minor isoform of glyceraldehyde-3-phosphate dehydrogenase. This increased expression could be reversed by the addition of acetoin, a NADH-specific redox sink, which furthermore largely restored anaerobic growth of the gpd2 strain. Additional deletion of the TDH1 gene (but not of TDH2 or TDH3) improved anaerobic growth of the gpd2 strain. We therefore propose that TDH1 has properties not displayed by the other TDH isogenes and that its expression is regulated by reductive stress caused by an excess of cytoplasmic NADH.

Place, publisher, year, edition, pages
Springer Verlag (Germany) , 2004. Vol. 45, no 2, 90-95 p.
National Category
Engineering and Technology
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URN: urn:nbn:se:liu:diva-72280DOI: 10.1007/s00294-003-0469-1ISI: 188748600004OAI: oai:DiVA.org:liu-72280DiVA: diva2:459042
Available from: 2011-11-24 Created: 2011-11-24 Last updated: 2017-12-08

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Ansell, Ricky

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