Dynamic crosstalk between developing blood cells and mesenchymal stroma compartments
(English)Manuscript (preprint) (Other academic)
The development of hematopoietic cells in the bone marrow is dependent on cellular interactions between blood cell progenitors and mesenchymal stroma cells. In order to increase the understanding of how cells communicate in this specialized environment, we have developed software scripts that allow us to compare gene expression patterns in two cells types and extract information about potential interaction pathways. The gene expression data was generated from freshly isolated FACS purified BM cells of hematopoietic or mesenchymal origins. This proposed that defined mesenchymal populations provide specific components to the microenvironment. Furthermore, even though several communication pathways were shared by multiple hematopoietic developmental stages, stage specific interactions may be involved in the modulation of defined progenitor populations. Additionally the analysis suggested that there existed possibilities for the hematopoietic cells to signal to the stroma cells and for the stroma cells to signal to each other. Our analysis suggests existence of a highly complex and dynamic crosstalk in the BM microenvironment.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-72334OAI: oai:DiVA.org:liu-72334DiVA: diva2:459201