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Variation in number of cagA EPIYA-C phosphorylation motifs between cultured Helicobacter pylori and biopsy strain DNA
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Surgery. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Surgery, Orthopaedics and Cancer Treatment, Department of Surgery in Östergötland.
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2012 (English)In: Infection, Genetics and Evolution, ISSN 1567-1348, E-ISSN 1567-7257, Vol. 12, no 1, 175-179 p.Article in journal (Refereed) Published
Abstract [en]

The Helicobacter pylori cagA gene encodes a cytotoxin which is activated by phosphorylation after entering the host epithelial cell. Phosphorylation occurs on specific tyrosine residues within EPIYA motifs in the variable 3'-region. Four different cagA EPIYA motifs have been defined according to the surrounding amino acid sequence; EPIYA-A, -B, -C and -D. Commonly, EPIYA-A and -B are followed by one or more EPIYA-C or -D motif. Due to observed discrepancies in cagA genotypes in cultured H. pylori and the corresponding DNA extracts it has been suggested that genotyping assays preferentially should be performed directly on DNA isolated from biopsy specimens. Gastric biopsies randomly selected from a Swedish cohort were homogenised and used for both direct DNA isolation and for H. pylori specific culturing and subsequent DNA isolation. In 123 of 153 biopsy specimens, the cagA EPIYA genotypes were in agreement with the corresponding cultured H. pylori strains. A higher proportion of mixed cagA EPIYA genotypes were found in the remaining 30 biopsy specimens. Cloning and sequencing of selected cagA EPIYA amplicons revealed variations in number of cagA EPIYA-C motifs in the mixed amplicons. The study demonstrates that culturing of H. pylori introduces a bias in the number of EPIYA-C motif. Consistent with other H. pylori virulence genotyping studies, we suggest that cagA EPIYA analysis should be performed using total DNA isolated from biopsy specimens.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 12, no 1, 175-179 p.
Keyword [en]
Gastroduodenal diseases; Helicobacter pylori; cagA EPIYA-C motif variation; Gastric biopsy H. pylori strains; Cultured H. pylori strains; Amplicon sequencing
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-72456DOI: 10.1016/j.meegid.2011.10.025ISI: 000299599600022PubMedID: 22085823OAI: oai:DiVA.org:liu-72456DiVA: diva2:459740
Note
funding agencies|Research council in the South-East of Sweden (FORSS)||ALF||Clinical Microbiology, Laboratory Centre-DC, University Hospital, Linkoping, Sweden||Available from: 2011-11-28 Created: 2011-11-28 Last updated: 2017-12-08

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Karlsson, AnneliRyberg, AnnaBorch, KurtMonstein, Hans-Jürg

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Karlsson, AnneliRyberg, AnnaBorch, KurtMonstein, Hans-Jürg
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Cell BiologyFaculty of Health SciencesClinical MicrobiologySurgeryDepartment of Surgery in ÖstergötlandDepartment of Clinical Microbiology
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Infection, Genetics and Evolution
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