Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids
2011 (English)In: Physical Review Letters, ISSN 0031-9007, Vol. 107, no 18, 188103- p.Article in journal (Refereed) Published
Glycosphingolipids are involved in the first steps of virus-cell interaction, where they mediate specific recognition of the host cell membrane. We have employed total-internal-reflection fluorescence microscopy to explore the interaction kinetics between individual unlabeled noroviruslike particles, which are attached to a glycosphingolipid-containing lipid bilayer, and fluorescent vesicles containing different types and concentrations of glycosphingolipids. Under association equilibrium, the vesicle-binding rate is found to be kinetically limited, yielding information on the corresponding activation energy. The dissociation kinetics are logarithmic over a wide range of time. The latter is explained by the vesicle-size-related distribution of the dissociation activation energy. The biological, pharmaceutical, and diagnostic relevance of the study is briefly discussed.
Place, publisher, year, edition, pages
American Physical Society , 2011. Vol. 107, no 18, 188103- p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-72658DOI: 10.1103/PhysRevLett.107.188103ISI: 000297004400007OAI: oai:DiVA.org:liu-72658DiVA: diva2:461133
Funding Agencies|Swiss National Science Foundation||VINNOVA||Swedish Foundation for Strategic Research||Swedish Research Council||2011-12-022011-12-022011-12-16