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Spermatozoa in the sperm-peak-fraction of the boar ejaculate show a lower flow of Ca(2+) under capacitation conditions post-thaw which might account for their higher membrane stability after cryopreservation
Swedish University of Agriculture Science SLU.
Swedish University of Agriculture Science SLU.
Swedish University of Agriculture Science SLU.
Swedish University of Agriculture Science SLU.
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2011 (English)In: Animal Reproduction Science, ISSN 0378-4320, E-ISSN 1873-2232, Vol. 128, no 1-4, 37-44 p.Article in journal (Refereed) Published
Abstract [en]

Boar spermatozoa collected in the ejaculate sperm peak-portion (P1, first 10 mL of the sperm-rich fraction, SRF), had shown a higher resilience to freezing and thawing compared to spermatozoa from the rest of the ejaculate (2nd portion of the SRF plus the post-sperm-rich fraction, PSRF), even when using a simplified freezing technique, as long as spermatozoa were incubated in their own seminal plasma (SP). This experiment studied the stability of P1- and SRF-P1 boar spermatozoa frozen in MiniFlatPacks (MFP), post-thaw, using flow cytometry. Since spermatozoa from either portion showed similar cryosurvival and low proportions of unstable membranes (less than3%, annexin-V/propidium iodide staining), and only a tendency for SRF-P1 live spermatozoa to depict acrosome exocytosis (FITC-PNA/PI/H33342); they were explored for Ca(2+) contents using a Fluo-4 probe under in vitro capacitating conditions (mBO+ medium), as well they were tested for their ability to sustain a short Ca(2+)-ionophore (A23187) in vitro challenge. The proportions of live spermatozoa depicting high Ca(2+)-levels were initially less than2% but increased over incubation time, particularly in SRF-P1(P less than 0.05), while proportions of live spermatozoa with low Ca(2+)-levels were basically constant over incubation time (similar to 11-14%), for either portion. Incubation in capacitation medium did not modify the proportions of low-Ca(2+) but dramatically increased the proportions of high-Ca(2+) spermatozoa (P less than 0.001) already after 15 min exposure, highest for SRF-P1 spermatozoa. While the proportion of live spermatozoa with intact acrosome was significantly decreased among SRF-P1 (P less than 0.001), that of P1-spermatozoa remained unchanged, probably owing to the lowest relative content of cytosolic Ca(2+). The results suggest that spermatozoa in the P1-portion are more resilient to express acrosome exocytosis post-thaw compared to those bathing in the rest of the SRF-fraction when cryopreserved using a simplified technique, in MFPs.

Place, publisher, year, edition, pages
Elsevier Masson , 2011. Vol. 128, no 1-4, 37-44 p.
Keyword [en]
Boar; Calcium; Membrane stability; Sperm; SRF
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-73348DOI: 10.1016/j.anireprosci.2011.08.006ISI: 000297892800005OAI: diva2:471362
Available from: 2012-01-02 Created: 2012-01-02 Last updated: 2015-03-12

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Rodriguez-Martinez, Heriberto
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