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Quantitative subproteomic analysis of age-related changes in mouse liver peroxisomes by iTRAQ LC-MS/MS
Stockholm University.
Uppsala University.
Uppsala University.
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.ORCID iD: 0000-0002-3894-2218
2011 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 879, no 30, 3393-3400 p.Article in journal (Refereed) Published
Abstract [en]

Aging is a complex multifactorial phenomenon, which is believed to result from the accumulation of cellular damage to biological macromolecules. Peroxisomes recently emerged as another important source of reactive oxygen species (ROS) production in addition to mitochondria. However, the role of these organelles in the process of aging is still not clear. The aim of this study was to characterize the changes in protein expression profiles of young (10 weeks old) versus old (18 months old) mouse liver peroxisome-enriched fractions. We have applied shotgun proteomic approach based on liquid chromatography and tandem mass spectrometry (LC-MS/MS) combined with iTRAQ (isobaric tags for relative and absolute quantitation) labeling that allows comparative quantitative multiplex analysis. Our analysis led to identification and quantification of 150 proteins, 8 out of which were differentially expressed between two age groups at a statistically significant level (p less than 0.05), with folds ranging from 1.2 to 4.1. These proteins involved in peroxisornal beta-oxidation, detoxification of xenobiotics and production of ROS. Noteworthy, differences in liver proteome have been observed between as well as within different age groups. In conclusion, our subproteomic quantitative study suggests that mouse liver proteome is sufficiently maintained until certain age.

Place, publisher, year, edition, pages
Elsevier , 2011. Vol. 879, no 30, 3393-3400 p.
Keyword [en]
Liquid chromatography; Tandem mass spectrometry; Peroxisomes; Aging; Liver; iTRAQ
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-73335DOI: 10.1016/j.jchromb.2011.08.044ISI: 000297396200006OAI: diva2:471870
Available from: 2012-01-03 Created: 2012-01-02 Last updated: 2015-03-24

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Cristobal, Susana
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Department of Clinical and Experimental MedicineFaculty of Health Sciences
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