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Initial development towards in vitro toxicity assay for lactate dehydrogenase using surface plasmon resonance
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, The Institute of Technology.
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Work with initial development towards in vitro toxicity assay for lactate dehydrogenase (LDH) using surface plasmon resonance is described. LDH is one of the important versatile biomarkers in in vitro hepatotoxicity. In this report LDH is detected by surface plasmon resonance using antibodies directly immobilized to the sensor surface. The assay is further extended with protein G immobilized to the surface to capture the antibody ligand. This has the advantage of simultaneously and on site purify and orient antibody ligand.

Keyword [en]
Lactate dehydrogenase, surface plasmon resonance, in vitro toxicity, biosensor, protein G
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-73409OAI: oai:DiVA.org:liu-73409DiVA: diva2:471881
Available from: 2012-01-03 Created: 2012-01-03 Last updated: 2012-01-03Bibliographically approved
In thesis
1. Exploring the SPR methodology for monitoring of critical attributes in toxicity testing and bioproduction
Open this publication in new window or tab >>Exploring the SPR methodology for monitoring of critical attributes in toxicity testing and bioproduction
2012 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.

A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.

When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.

The design of a sensor surface is important for the characteristics of a sensor. By binding antibodies in an oriented manner to the surface a better control of the properties of the antibodies is achieved. The demonstrated method also has the advantage of in situ purification and provides a flexible platform for antibody evaluation and sensor development.

In one sentence this thesis explores the possibility of utilizing recognition elements of a biosensor surface. In particular, surface plasmon resonance (SPR) is used as the primary biosensing tool, however most findings in are relevant for other biosensors.

Moreover, the thesis approaches existing bioanalytical impediments, such as purity and accessibility of the recognition elements on the sensor surface and preparation strategies to achieve this.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2012. 19 p.
Series
Linköping Studies in Science and Technology. Thesis, ISSN 0280-7971 ; 1517
National Category
Natural Sciences
Identifiers
urn:nbn:se:liu:diva-73410 (URN)LIU-TEK-LIC-2012:2 (Local ID)978-91-7519-971-9 (ISBN)LIU-TEK-LIC-2012:2 (Archive number)LIU-TEK-LIC-2012:2 (OAI)
Presentation
2012-01-31, Panck, Fysikhuset, Campus Valla, Linköpings Universitet, Linköping, 09:15 (Swedish)
Opponent
Supervisors
Available from: 2012-01-03 Created: 2012-01-03 Last updated: 2012-01-11Bibliographically approved

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Bergström, GunnarMandenius, Carl-Fredrik

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Citation style
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