liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
PNAS-4 expression and its relationship to p53 in colorectal cancer
Sichuan University.
Sichuan University.
Sichuan University.
Sichuan University.
Show others and affiliations
2012 (English)In: Molecular Biology Reports, ISSN 0301-4851, E-ISSN 1573-4978, Vol. 39, no 1, 243-249 p.Article in journal (Refereed) Published
Abstract [en]

PNAS-4 is a novel pro-apoptotic protein activated during the early response to DNA damage; however, the molecular mechanisms and pathways regulating PNAS-4 expression in tumors are not well understood. We hypothesized that PNAS-4 is a p53 down-stream target gene and designed this study. We searched online for putative p53-binding sites in the entire PNAS-4 gene and did not find any corresponding information. In HCT116 colon cancer cells, after being transfected with small interfering RNA to silence p53, the expressions of PNAS-4 and other known p53 target gene (Apaf1, Bax, Fas and Dr5) were determined by real-time PCR. We found that PNAS-4 was up-regulated while Apaf1, Bax, Fas and Dr5 were down-regulated. We then examined the expression of PNAS-4 and p53 mutation in colorectal cancer patients. PNAS-4 expressed both in colorectal cancers and normal tissues, but compared with paired control, PNAS-4 was up-regulated in cancers (P = 0.018). PNAS-4 overexpression ratios were correlated to the p53 mutant status (P = 0.001). The mean PNAS-4 expression levels of p53 mutant homozygote group and heterozygote group were higher than that of p53 wild type group (P = 0.013). The expression ratios of PNAS-4 (every sample in relative to its paired normal mucosa) were different between negative lymph node metastasis (66% up-regulated, 34% down-regulated) and positive metastasis (42% up-regulated, 58% down-regulated). Taken together, these findings suggested that PNAS-4 was not a p53 target, but overexpression of PNAS-4 was correlated to p53 inactivity in colorectal cancer.

Place, publisher, year, edition, pages
Springer Verlag (Germany) , 2012. Vol. 39, no 1, 243-249 p.
Keyword [en]
Colorectal carcinoma; PNAS-4; Small interfering RNA; p53; Real-time PCR
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-73308DOI: 10.1007/s11033-011-0732-3ISI: 297355700031OAI: diva2:472112
Available from: 2012-01-03 Created: 2012-01-02 Last updated: 2012-01-03

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Sun, Xiao-Feng
By organisation
OncologyFaculty of Health SciencesDepartment of Oncology UHL
In the same journal
Molecular Biology Reports
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 48 hits
ReferencesLink to record
Permanent link

Direct link