Quantification of indoxyl sulphate in the spent dialysate using fluorescence spectra
2011 (English)In: IFMBE Proceedings, ISSN 1680-0737, Vol. 34, 45-48 p.Article in journal (Refereed) Published
The aim of this study was to investigate the possibility to determine the amount of Indoxyl Sulphate (IS) in the spent dialysate using fluorescence spectra. Eight uremic patients from Linköping were studied during their three dialysis treatments in one week at the Department of Dialysis and Nephrology at Linköping University Hospital. Dialysate samples were taken during each treatment and analyzed, IS concentration was estimated using HPLC method, and fluorescence spectra was measured with spectrofluorophotometer. The fluorescence spectral values were transformed into IS concentration using regression model from total material noted as fluorescence method (F). Achieved results were compared regarding mean values and SD. Mean value of IS estimated by HPLC was 1.21±0.77 mg/l and by F 1.22±0.72 mg/l. Concentrations were not significantly different (p≤0,05). This study indicates, that it is possible to estimate the concentration of IS using only fluorescence values of the spent dialysate.
Place, publisher, year, edition, pages
2011. Vol. 34, 45-48 p.
Indoxyl Sulphate – dialysis – fluorescence – spectra – uremic toxins
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-73440DOI: 10.1007/978-3-642-21683-1_11OAI: oai:DiVA.org:liu-73440DiVA: diva2:472459
15th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC 2011) 2012-01-032012-01-032012-01-11