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Polypeptide Folding-Mediated Tuning of the Optical and Structural Properties of Gold Nanoparticle Assemblies
University of London Imperial College of Science Technology and Medicine.ORCID iD: 0000-0002-7001-9415
University of London Imperial College of Science Technology and Medicine.
Research Centre Nanosci and Nanotechnol CIN2 CSIC.
University of London Imperial College of Science Technology and Medicine.
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2011 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 11, no 12, 5564-5573 p.Article in journal (Refereed) Published
Abstract [en]

Responsive hybrid nanomaterials with well-defined properties are of significant interest for the development of biosensors with additional applications in tissue engineering and drug delivery. Here, we present a detailed characterization using UV-vis spectroscopy and small angle X-ray scattering of a hybrid material comprised of polypeptide-decorated gold nanoparticles with highly controllable assembly properties. The assembly is triggered by a folding-dependent bridging of the particles mediated by the heteroassociation of immobilized helix-loop-helix polypeptides and a complementary nonlinear polypeptide present in solution. The polypeptides are de novo designed to associate and fold into a heterotrimeric complex comprised of two disulfide-linked four-helix bundles. The particles form structured assemblies with a highly defined interparticle gap (4.8 +/- 0.4 nm) that correlates to the size of the folded polypeptides. Transitions in particle aggregation dynamics, mass-fractal dimensions and ordering, as a function of particle size and the concentration of the bridging polypeptide, are observed; these have significant effects on the optical properties of the assemblies. The assembly and ordering of the particles are highly complex processes that are affected by a large number of variables including the number of polypeptides bridging the particles and the particle mobility within the aggregates. A fundamental understanding of these processes is of paramount interest for the development of novel hybrid nanomaterials with tunable structural and optical properties and for the optimization of nanoparticle-based colorimetric biodetection strategies.

Place, publisher, year, edition, pages
American Chemical Society , 2011. Vol. 11, no 12, 5564-5573 p.
Keyword [en]
Gold nanoparticles; polypeptides; folding; helix-loop-helix; small angle X-ray scattering (SAXS)
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-73736DOI: 10.1021/nl203559sISI: 000297950200080OAI: diva2:476396
Available from: 2012-01-12 Created: 2012-01-12 Last updated: 2014-10-08

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