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RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development
Lund University.
Lund University.
Lund University.
Lund University.
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2003 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 74, no 1, 102-110 p.Article in journal (Refereed) Published
Abstract [en]

The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell fines representing defined stages of B cell development using Affymetrix(TM) microarrays. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell. lines was established. The data indicated that the cell lines representing a defined stage generally presented a high similarity in overall expression profiles. Numerous genes could be identified as expressed with a restricted pattern using dCHIP-based, quantitative comparisons or presence/absence-based, probabilistic state analysis. These experiments provide a model for gene expression during B cell development, and the correctly identified expression patterns of a number of control genes suggest that a series of cell fines can be useful tools in the elucidation of the molecular genetics of a complex differentiation process.

Place, publisher, year, edition, pages
Society for Leukocyte Biology , 2003. Vol. 74, no 1, 102-110 p.
Keyword [en]
gene expression; immunoglobulin; progenitor B cells
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-74463DOI: 10.1189/jlb.0103008ISI: 000187367800014OAI: diva2:484758
Available from: 2012-01-27 Created: 2012-01-27 Last updated: 2012-02-06

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Sigvardsson, Mikael
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