RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development
2003 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 74, no 1, 102-110 p.Article in journal (Refereed) Published
The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell fines representing defined stages of B cell development using Affymetrix(TM) microarrays. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell. lines was established. The data indicated that the cell lines representing a defined stage generally presented a high similarity in overall expression profiles. Numerous genes could be identified as expressed with a restricted pattern using dCHIP-based, quantitative comparisons or presence/absence-based, probabilistic state analysis. These experiments provide a model for gene expression during B cell development, and the correctly identified expression patterns of a number of control genes suggest that a series of cell fines can be useful tools in the elucidation of the molecular genetics of a complex differentiation process.
Place, publisher, year, edition, pages
Society for Leukocyte Biology , 2003. Vol. 74, no 1, 102-110 p.
gene expression; immunoglobulin; progenitor B cells
National CategoryEngineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-74463DOI: 10.1189/jlb.0103008ISI: 000187367800014OAI: oai:DiVA.org:liu-74463DiVA: diva2:484758