liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development
Lund University.
Lund University.
Lund University.
Lund University.
Show others and affiliations
2003 (English)In: Journal of Leukocyte Biology, ISSN 0741-5400, E-ISSN 1938-3673, Vol. 74, no 1, 102-110 p.Article in journal (Refereed) Published
Abstract [en]

The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell fines representing defined stages of B cell development using Affymetrix(TM) microarrays. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell. lines was established. The data indicated that the cell lines representing a defined stage generally presented a high similarity in overall expression profiles. Numerous genes could be identified as expressed with a restricted pattern using dCHIP-based, quantitative comparisons or presence/absence-based, probabilistic state analysis. These experiments provide a model for gene expression during B cell development, and the correctly identified expression patterns of a number of control genes suggest that a series of cell fines can be useful tools in the elucidation of the molecular genetics of a complex differentiation process.

Place, publisher, year, edition, pages
Society for Leukocyte Biology , 2003. Vol. 74, no 1, 102-110 p.
Keyword [en]
gene expression; immunoglobulin; progenitor B cells
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-74463DOI: 10.1189/jlb.0103008ISI: 000187367800014OAI: diva2:484758
Available from: 2012-01-27 Created: 2012-01-27 Last updated: 2012-02-06

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Sigvardsson, Mikael
In the same journal
Journal of Leukocyte Biology
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 4 hits
ReferencesLink to record
Permanent link

Direct link