Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics
2005 (English)In: Physiological Genomics, ISSN 1094-8341, E-ISSN 1531-2267, Vol. 23, no 2, 206-216 p.Article in journal (Refereed) Published
Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency.
Place, publisher, year, edition, pages
American Physiological Society , 2005. Vol. 23, no 2, 206-216 p.
adipogenesis; early B cell factor; peroxisome proliferator-activated receptor-gamma 2
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-74450DOI: 10.1152/physiolgenomics.00015.2005ISI: 000234086200009OAI: oai:DiVA.org:liu-74450DiVA: diva2:484809